DNA participating in hydroxylation of macrolide compound
View Patent ↗The present invention provides a DNA participating in biological transformation of a macrolide compound 11107B. The present invention provides, particularly, a DNA participating in biological transformation of a macrolide compound 11107B represented by the formula (I) into a 16-position hydroxy macrolide compound 11107D represented by the formula (II), the DNA encoding a protein having 16-position hydroxylating enzymatic activity or ferredoxin, to a method of isolating the DNA, to a protein encoded by the DNA, a plasmid carrying the DNA, a transformant obtained by transforming using the plasmid and a method of producing a 16-position hydroxy compound by using the transformant.
1. A DNA participating in biological transformation of a macrolide compound (hereinafter referred to as a macrolide compound 11107B) represented by the formula (I):
into a 16-position hydroxy macrolide compound represented by the formula (II):
the DNA being an isolated and pure DNA comprising a DNA encoding a protein having
16-position hydroxylating enzymatic activity which is characterized by the following (a), (b), or (c):
(a) a DNA encoding a protein having the enzymatic activity to hydroxylate the 16-position of the macrolide compound 11107B, wherein the DNA is selected from the group consisting of (1) a continuous nucleotide sequence from base 1322 to base 2548 of SEQ ID NO: 1; (2) a continuous nucleotide sequence from base 420 to base 1604 of SEQ ID NO: 4; and (3) a continuous nucleotide sequence from base 172 to base 1383 of SEQ ID NO: 7;
(b) a DNA which has a nucleotide sequence having 90% or more identity over the full length sequence with the DNA described in (a);
(c) a DNA encoding a protein having the same amino acid sequence as the protein encoded by the DNA described in (a) or (b) though it does not have 90% or more identity with the DNA described in (a) because of the degeneracy of a gene codon.
2. A self-replicative or integrating replicative recombinant plasmid carrying the DNA as claimed in claim 1 .
3. An isolated transformant obtained by transformation of the recombinant plasmid of claim 2 .
4. The DNA according to claim 1 , wherein the DNA comprises bases 1322-2548 of SEQ ID NO: 1.
5. The DNA according to claim 1 , wherein the DNA encodes a polypeptide comprising SEQ ID NO: 2.
6. The DNA according to claim 1 , wherein the DNA consists of bases 1322-2548 of SEQ ID NO: 1.
7. The DNA according to claim 1 , wherein the DNA encodes a polypeptide consisting of SEQ ID NO: 2.
8. The DNA according to claim 1 , wherein said identity in (b) and (c) of claim 1 is 95% or more.
9. A DNA comprising
(a) a DNA encoding a protein, wherein the DNA is selected from the group consisting of (1) a continuous nucleotide sequence from base 1322 to base 2548 of SEQ ID NO: 1; (2) a continuous nucleotide sequence from base 420 to base 1604 of SEQ ID NO: 4; and a continuous nucleotide sequence from base 172 to base 1383 of SEQ ID NO: 7;
(b) a DNA which has a nucleotide sequence having 90% or more identity over the full length sequence with the DNA described in (a); or
(c) a DNA encoding a protein having the same amino acid sequence as the protein encoded by the DNA described in (a) or (b) though it does not have 90% or more identity with the DNA described in (a) because of the degeneracy of a gene codon.
10. The DNA according to claim 9 , wherein said identity in (b) and (c) of claim 9 is 95% or more.