Commercially viable process for in vitro mass culture of
The present invention relates to a commercially viable process for in vitro mass culture of Jatropha curcas . The process for in vitro mass culture of Jatropha curcas is simple, faster, and suitable for production of disease-free root tubers of uniform quality and employs media with a reduced concentration of phytohormones.
1. A method for producing a true-to-type clone of a Jatropha curcas mother plant comprising culturing a meristematic explant of Jatropha curcas in media with phytohormones at a concentration from 0.01 mg/L to 0.3 mg/L, wherein said phytohormones are cytokinins, and producing a true-to-type clone of a Jatropha curcas mother plant from said meristematic explant.
2. The method of claim 1 , wherein said meristematic explant is from a shoot tip or a nodal bud.
3. The method of claim 2 , wherein said meristematic explant is from a shoot tip.
4. The method of claim 3 , wherein said shoot tip comprises bud tissue.
5. The method of claim 4 , wherein said bud tissue is apical bud tissue.
6. The method of claim 1 , wherein said phytohormones are at concentration selected from the group consisting of 0.01 mg/L, 0.1 mg/L, and 0.3 mg/L.
7. The method of claim 1 , wherein said cytokinin is 6-benzyl aminopurine.
8. The method of claim 7 , wherein said 6-benzyl aminopurine is at a concentration of 0.44 μM.