Lignan glycosidase and utilization of the same
The present invention provides an enzyme having the lignan glycosidation activity by identifying the enzyme that is involved in the production of lignan glycosides, identifying the amino acid sequence of the enzyme polypeptide and the base sequence for a polynucleotide encoding the polypeptide, and based on the information of these sequences, preparing the transformants capable of producing the lignan glycosides.
1. An isolated polynucleotide encoding a polypeptide having a lignan glycosidation activity, which is either (a) or (b):
(a) a polynucleotide consisting of the nucleotide sequence of any one of SEQ ID NO: 1, 81, or 90; or
(b) a polynucleotide consisting of a nucleotide sequence which has at least 90% sequence identity to SEQ ID NO: 1, 81, or 90.
2. A vector comprising the polynucleotide of claim 1 .
3. A method for producing a polypeptide having lignan glycosidation activity, which comprises:
(a) transforming a host cell with the vector of claim 2 ;
(b) culturing the host cell; and
(c) isolating the polypeptide from the cell culture.
4. An isolated host cell transformed with the polynucleotide of claim 1 .
5. The host cell-according to claim 4 , wherein content ratios of lignan and a lignan glycoside are modified.
6. A method for producing a polypeptide having lignan glycosidation activity, which comprises culturing the host cell of claim 4 and isolating the polypeptide from the cell culture.
7. An isolated cell comprising the vector according to claim 2 .
8. The isolated polynucleotide of claim 1 , wherein in (b) the polynucleotide consists of a nucleotide sequence which has at least 95% sequence identity to SEQ ID NO: 1, 81, or 90.