Spinach line SMB66-1082F
The invention provides seed and plants of the spinach line designated SMB66-1082F. The invention thus relates to the plants, seeds and tissue cultures of spinach line SMB66-1082F, and to methods for producing a spinach plant produced by crossing a plant of spinach line SMB66-1082F with itself or with another spinach plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of spinach line SMB66-1082F, including the fruit and gametes of such plants.
1. A seed of spinach line SMB66-1082F a sample of seed of said line having been deposited under ATCC Accession Number PTA-9188.
2. A plant grown from the seed of claim 1 .
3. A plant part of the plant of claim 2 .
4. The plant part of claim 3 , wherein said part is selected from the group consisting of a seed, pollen, an ovule, a leaf, and a cell.
5. A spinach plant, or a part thereof, having all the physiological and morphological characteristics of the spinach plant of claim 2 .
6. A tissue culture of regenerable cells of spinach line SMB66-1082F, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9188.
7. The tissue culture according to claim 6 , comprising cells or protoplasts from a plant part selected from the group consisting of embryos, meristems, cotyledons, pollen, leaves, anthers, roots, root tips, shoots, pistil, flower, seed and stalks.
8. A spinach plant regenerated from the tissue culture of claim 6 , wherein the regenerated plant expresses all of the physiological and morphological characteristics of spinach line SMB66-1082F, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9188.
9. A method of producing seed, comprising crossing the plant of claim 2 with itself or a second spinach plant and allowing seed to form.
10. The method of claim 9 , comprising crossing the plant of spinach line SMB66-1082F with a spinach plant of a different genotype relative to said line.
11. An F1 hybrid seed produced by the method of claim 10 .
12. An F1 hybrid plant produced by growing the seed of claim 11 .
13. A method for producing a seed of a line SMB66-1082F-derived spinach plant comprising the steps of:
(a) crossing a spinach plant of line SMB66-1082F, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9188, with a second spinach plant; and
(b) allowing seed of a SMB66-1082F-derived spinach plant to form.
14. The method of claim 13 , further comprising the steps of:
(c) crossing a plant grown from said SMB66-1082F-derived spinach seed with itself or a second spinach plant to yield additional SMB66-1082F-derived spinach seed;
(d) growing said additional SMB66-1082F-derived spinach seed of step (c) to yield additional SMB66-1082F-derived spinach plants; and
(e) repeating the crossing and growing steps of (c) and (d) to generate further SMB66-1082F-derived spinach plants.
15. A method of vegetatively propagating a plant of spinach line SMB66-1082F comprising the steps of:
(a) collecting tissue capable of being propagated from a plant of spinach line SMB66-1082F, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9188;
(b) cultivating said tissue to obtain proliferated shoots; and
(c) rooting said proliferated shoots to obtain rooted plantlets.
16. The method of claim 15 , further comprising growing plants from said rooted plantlets.
17. A method of introducing a desired trait into spinach line SMB66-1082F comprising:
(a) crossing a plant of line SMB66-1082F, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9188, with a second spinach plant that comprises a desired trait to produce F1 progeny;
(b) selecting an F1 progeny that comprises the desired trait;
(c) crossing the selected F1 progeny with a plant of line SMB66-1082F to produce backcross progeny;
(d) selecting backcross progeny comprising the desired trait and the physiological and morphological characteristic of spinach line SMB66-1082F; and
(e) repeating steps (c) and (d) three or more times in succession to produce selected fourth or higher backcross progeny that comprise the desired trait.
18. A spinach plant produced by the method of claim 17 , wherein the spinach plant comprises essentially all of the physiological and morphological characteristics of spinach line SMB66-1082F, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9188.
19. A method of producing a plant of spinach line SMB66-1082F, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9188, comprising an added desired trait, the method comprising introducing a transgene conferring the desired trait into a plant of spinach line SMB66-1082F to produce a plant of spinach line SMB66-1082F comprising an added desired trait.
20. A progeny plant of the plant of claim 2 that comprises all of the physiological and morphological characteristics of spinach line SMB66-1082F, a sample of seed of said line having been deposited under ATCC Accession Number PTA-9188.
21. A seed that produces the plant of claim 20 .
22. A method of determining the genotype of the plant of claim 2 , comprising obtaining a sample of nucleic acids from said plant and detecting in said nucleic acids a plurality of polymorphisms.
23. The method of claim 22 , further comprising the step of storing the results of the step of detecting the plurality of polymorphisms on a computer readable medium.
24. A method of producing spinach comprising:
(a) obtaining the plant of claim 2 , and
(b) collecting leaf tissue from the plant.