Treatment of transformed or infected biological cells
View Patent ↗The present invention relates to a therapeutic or/and diagnostic substance. Furthermore it relates to an expression vector, to a composition comprising the afore-mentioned substance or/and the afore-mentioned expression vector, a method for diagnosing a tumor disease or/and an infectious disease in a living being, as well as to a method for the treatment of a tumor disease or/and of an infection in a living being.
1. Method for diagnosing a tumor disease and/or an infection in a living being in vitro, comprising:
(a) providing leucocytes to be analyzed from said living being,
(b) analyzing in said leucocytes the phosphorylation state of a retinoblastoma protein involved in the regulation of the cell cycle and the phosphorylation state of a MAP kinase involved in the regulation of the cell cycle, wherein said retinoblastoma protein is involved in the regulation of the cell cycle at a first time in normal cells and said MAP kinase is involved in the regulation of the cell cycle at a second time in normal cells, and
(c) correlating the finding of simultaneous phosphorylation states of said retinoblastoma protein and said MAP kinase in said leucocytes with a positive diagnosis.
2. Method for diagnosing a tumor disease and/or an infection in a living being in vitro, comprising:
(a) providing single cells to be analyzed from said living being,
(b) analyzing in said single cells the activity of a substrate of the CDK2 signal pathway involved in the regulation of the cell cycle and the activity of a substrate of the ras/raf signal pathway involved in the regulation of the cell cycle, wherein said substrate of the CDK2 signal pathway is involved in the regulation of the cell cycle at a first time in normal cells and said substrate of the ras/raf signal pathway is involved in the regulation of the cell cycle at a second time in normal cells, and
(c) correlating the finding of simultaneous activities of said substrate of the CDK2 signal pathway and said substrate of the ras/raf signal pathway in said single cells with a positive diagnosis.
3. Method according to claim 2 , wherein:
(i) the substrate of the CDK2 signal pathway is a retinoblastoma protein; and,
(ii) the substrate of the ras/raf signal pathway is a MAP kinase.
4. Method according to claim 3 , wherein:
(i) the activity of the retinoblastoma protein is the phosphorylation state of the retinoblastoma protein; and,
(ii) the activity of the MAP kinase is the phosphorylation state of the MAP kinase.
5. Method according to claim 2 , wherein the single cells are leucocytes.