Imaging and evaluating embryos, oocytes, and stem cells
View Patent ↗Methods, compositions and kits for determining the developmental potential of one or more embryos or pluripotent cells and/or the presence of chromosomal abnormalities in one or more embryos or pluripotent cells are provided. These methods, compositions and kits find use in identifying embryos and oocytes in vitro that are most useful in treating infertility in humans.
1. A method for assessing the potential for developmental competence of a human embryo comprising
1) measuring cellular parameters of human embryo in vitro, wherein said cellular parameters include:
(a) the duration of the first cytokinesis;
(b) the time interval between cytokinesis 1 and cytokinesis 2; or
(c) the time interval between cytokinesis 2 and cytokinesis 3, and
2) determining that the human embryo has greater potential for developmental competence when,
(a′) the duration of the first cytokinesis is about 0 to about 30 minutes;
(b′) the time interval between the resolution of cytokinesis 1 and the onset of cytokinesis 2 is about 8-15 hours; or
(c′) the time interval between the onset of cytokinesis 2 and the onset of cytokinesis 3 is about 0-5 hours.
2. The method of claim 1 wherein the cellular parameters include:
(a) the duration of the first cytokinesis;
(b) the time interval between cytokinesis 1 and cytokinesis 2; and
(c) the time interval between cytokinesis 2 and cytokinesis 3.
3. The method of claim 1 , wherein said cellular parameters are measured by time-lapse microscopy.
4. The method of claim 1 , wherein said cellular parameters further comprise the duration of cell cycle 1.
5. The method of claim 4 , wherein greater potential for developmental competence of said human embryo is indicated when the duration of cell cycle 1 is about 20 to about 27 hours.
6. The method of claim 1 , wherein poor developmental potential of said human embryo is indicated by
(a) a longer cytokinesis for said human embryo than for said reference human embryo;
(b) a longer or shorter time interval between the resolution of cytokinesis 1 and the onset of cytokinesis 2 for said human embryo than for said reference human embryo; and/or
(c) a longer time interval between the initiation of cytokinesis 2 and the onset of cytokinesis 3 for said human embryo than for said reference human embryo.
7. The method of claim 6 , wherein said cellular parameters further comprise the duration of cell cycle 1.
8. The method of claim 7 , wherein poor developmental competence of said human embryo is indicated when the duration of cell cycle 1 is longer than about 27 hours.
9. The method of claim 1 , wherein said embryos are produced by fertilization of oocytes in vitro.
10. The method of claim 1 , wherein said oocytes are matured in vitro.
11. The method of claim 10 , wherein said oocytes matured in vitro are supplemented with growth factors.
12. The method of claim 1 , wherein the embryos have not been frozen prior to measuring the parameters.
13. The method of claim 1 , wherein the embryos have been frozen prior to measuring the parameters.
14. The method of claim 1 further comprising implanting the human embryo determined to have greater potential for developmental competence into a female human subject.