Methods and compositions for immunizing against
View Patent ↗The present invention relates, in part, to methods and compositions for immunizing against infection by Chlamydia trachomatis . The methods and compositions rely, in part, on administering an immunogenic composition comprising one or more peptides derived from C. trachomatis major outer membrane protein (MOMP) to a subject to be immunized. In some embodiments, the compositions comprise a chimeric immunogen comprising a receptor binding domain, a translocation domain, and a Chlamydia trachomatis antigen. Polynucleotides encoding the chimeric immunogens, expression vectors comprising the polynucleotides, and kits comprising the compositions are also provided.
1. A chimeric immunogen, comprising
a) a Pseudomonas exotoxin A (PE) receptor binding domain Ia
b) a PE translocation domain II,
c) a Chlamydia trachomatis antigen selected from the group consisting of AETIFDVTTLNPTIAGAGDVKTSAEG (SEQ ID NO.:6), AETILDVTTLNPTIAGKGTVVTSAE (SEQ ID NO.:7), TTLNPTIAGC (SEQ ID NO.:8), CAETIFDVTTLNPTIAGAGDVKTSAEGC (SEQ ID NO.: 15), CAETILDVTTLNPTIAGKGTVVTSAEC (SEQ ID NO.:16), and CTTLNPTIAGC (SEQ ID NO.:17) inserted into PE domain Ib, and
d) a PE endoplasmic reticulum retention domain III, wherein the PE domain Ib is located between PE translocation domain II and PE endoplasmic reticulum retention domain III wherein the said chimeric immunogen does not comprise more than one C. trachomatis antigen.
2. The chimeric immunogen of claim 1 , wherein said C. trachomatis antigen replaces all or a portion of said PE domain Ib.
3. The chimeric immunogen of claim 1 , wherein said PE endoplasmic reticulum retention domain III is an enzymatically inactive PE domain III.
4. The chimeric immunogen of claim 3 , wherein said enzymatically inactive PE domain III is inactivated by deleting a glutamate at position 553.
5. The chimeric immunogen of claim 1 , wherein said PE endoplasmic reticulum retention domain III comprises an amino acid sequence that is selected from the group of RDEL (SEQ ID NO.:20) or KDEL (SEQ ID NO.:21).
6. The chimeric immunogen of claim 1 , wherein said translocation domain comprises amino acids 280 to 364 of domain II of Pseudomonas exotoxin A.
7. A composition comprising the chimeric immunogen of claim 1 .
8. The composition of claim 7 , wherein said composition further comprises a pharmaceutically acceptable diluent, excipient, vehicle, or carrier.
9. A kit, comprising an article of manufacture containing the composition claim 7 .