IP Library › Granted Patent US 7,982,029
Granted Patent B2
US 7,982,029 · App. 12/084,338 · Granted Jul 19, 2011

Synthesis of four color 3′O-allyl, modified photocleavable fluorescent nucleotides and related methods

Assignee: The Trustees of Columbia University in the City of New York
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Quick Facts
Patent No.
US 7,982,029
App. No.
12/084,338
Granted
Jul 19, 2011
Kind
B2
Abstract

This invention provides a process for making 3′-O-allyl-dGTP-PC-Biodopy-FL-510, 3′-O-allyl-dATP-PC-ROX, 3′-O-allyl-dCTP-PC-Bodipy-650 and 3′-O-allyl-dUTP-PC-R6G, and related methods.

Claims (4)

1. A method for making 3′O-allyl-dGTP-PC-Bodipy-FL-510 comprising performing the steps set forth in FIG. 7 .

2. A method for making 3′-O-allyl-dATP-PC-ROX comprising performing the steps set forth in FIG. 8 .

3. A method for making 3′-(D-allyl-dCTP-PC-Bodipy-650 comprising performing the steps set forth in FIG. 9 .

4. A method for making 3′-O-allyl-dUTP-PC-R6G comprising performing the steps set forth in FIG. 10 .

Assignments (2)
CONFIRMATORY LICENSE Recorded Jan 12, 2012
From: COLUMBIA UNIV NEW YORK MORNINGSIDE
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 027522/0549 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 29, 2008
From: JU, JINGYUE; MENG, QINGLIN; KIM, DAE H.; BI, LANRONG; BAI, XIAOPENG; TURRO, NICHOLAS J.
To: TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK, THE
Reel/Frame 022055/0455 →
Continuity (2)
Provisional Application 60732373 · Oct 31, 2005
Related Publication 20100041029A1 · Feb 18, 2010