Gene encoding lignan methylation enzyme
The present invention relates to genes for enzymes having the activity of transferring a methyl group to lignans, plants with an altered lignan composition using these methyltransferases, and so on. More particularly, the present invention relates to enzyme genes having the activity of synthesizing methylated lignans, preferably enzyme genes having the activity of synthesizing sesame-derived methylated lignans, and use thereof.
1. An isolated polynucleotide as defined in any one of (a) through (d) below:
(a) an isolated polynucleotide comprising a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 1;
(b) an isolated polynucleotide comprising a polynucleotide encoding a protein consisting of the amino acid sequence of SEQ ID NO: 2;
(c) an isolated polynucleotide consisting of a nucleotide sequence having at least 95% identity to SEQ ID NO:1, wherein the polynucleotide encodes a protein having the activity of transferring a methyl group to a lignan; and,
(d) an isolated polynucleotide comprising a polynucleotide encoding a protein consisting of the amino acid sequence that has been modified by substitution, deletion, insertion, and/or addition of one to six amino acids of SEQ ID NO: 2 and having an activity of transferring a methyl group to a lignan.
2. The polynucleotide according to claim 1 , which comprises a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 1.
3. The polynucleotide according to claim 1 , which comprises a polynucleotide encoding a protein consisting of the amino acid sequence of SEQ ID NO: 2.
4. The polynucleotide according to claim 1 , which is a DNA.
5. The polynucleotide according to claim 1 , which encodes a protein having an activity of transferring a methyl group to a furofuran lignan.
6. The polynucleotide according to claim 5 , which encodes a protein having an activity of transferring a methyl group to pinoresinol and/or piperitol.
7. An isolated protein encoded by the polynucleotide according to claim 1 .
8. A vector comprising the polynucleotide according to claim 1 .
9. A host cell transformed by the vector according to claim 8 .
10. A method of producing a protein having an activity of transferring a methyl group to a lignan, which comprises culturing or growing the host cell according to claim 9 and collecting said protein from said host cell.
11. A plant transformed with the polynucleotide according to claim 1 , or a plant which is a progeny of said plant having the same properties as the plant, or a tissue of these plants.
12. A method of transferring a methyl group to a lignan, which comprises using the polynucleotide according to claim 1 .
13. A plant with an altered lignan composition produced by transformation and expression of said plant with the polynucleotide according to claim 1 , or a plant which is a progeny of the plant having the same properties as the plant.