Nucleic acids encoding ChemerinR ligands
The present invention relates to a G-protein coupled receptor and a novel ligand therefor. The invention provides screening assays for the identification of candidate compounds which modulate the activity of the G-protein coupled receptor, as well as assays useful for the diagnosis and treatment of a disease or disorder related to the dysregulation of G-protein coupled receptor signaling.
1. An isolated nucleic acid molecule encoding the polypeptide sequence of SEQ ID NO: 14.
2. The isolated nucleic acid molecule of claim 1 , wherein said nucleic acid molecule consists of the sequence of SEQ ID NO: 13.
3. An isolated nucleic acid molecule encoding a Chemerin polypeptide that specifically binds to a ChemerinR polypeptide of SEQ ID NO: 2, wherein the nine C-terminal amino acids of said Chemerin polypeptide are set forth in SEQ ID NO: 61.
4. The isolated nucleic acid molecule of claim 3 , wherein the sequence of said Chemerin polypeptide is set forth in SEQ ID NO: 14.
5. The isolated nucleic acid molecule of claim 3 , wherein the sequence of said Chemerin polypeptide is set forth in SEQ ID NO: 53.
6. The isolated nucleic acid molecule of claim 3 , wherein said nucleic acid molecule comprises the sequence of SEQ ID NO: 13.
7. An isolated host cell comprising a recombinant nucleic acid molecule encoding the polypeptide sequence of SEQ ID NO: 14.
8. A method for producing the polypeptide of SEQ ID NO: 14 comprising culturing the host cell of claim 7 under conditions wherein said recombinant nucleic acid molecule is expressed and recovering the polypeptide encoded by said isolated nucleic acid molecule.
9. An isolated host cell comprising a recombinant nucleic acid molecule encoding a Chemerin polypeptide that specifically binds to the ChemerinR polypeptide of SEQ ID NO: 2, wherein the nine C-terminal amino acids of said Chemerin polypeptide are set forth in SEQ ID NO: 61.
10. A method for producing a Chemerin polypeptide, said method comprising culturing the host cell of claim 9 under conditions wherein said recombinant nucleic acid molecule is expressed and recovering the polypeptide encoded by said isolated nucleic acid molecule.