Slitrks as markers for stem and progenitor cells and methods of use thereof
The present invention relates to slitrk proteins as markers of stem and progenitor cells, including embryonic stem cells and hematopoietic stem and progenitor cells, and also as a marker of leukemia and lymphoma cells, and of endothelial cells. The invention provides, inter alia, methods for purifying slitrk-positive cells, methods for detecting slitrk-positive cells, purified preparations of slitrk-positive cells, therapeutic compositions containing purified slitrk-positive cells, methods for targeting therapeutic agents to slitrk-positive cells, and methods of treatment, including but not limited to, methods of administering slitrk-positive cells to subjects in need thereof.
1. A method for detecting stem or progenitor cells in a tissue, a tissue sample or a cell population, comprising:
(a) obtaining a tissue, a tissue sample or a cell population, and
(b) determining whether the tissue, the tissue sample or the cell population contains slitrk4 mRNA, wherein the determining step comprises contacting the tissue, the tissue sample or the cell population with a primer or probe that binds to slitrk4 mRNA, and
(c) correlating the presence of slitrk4 mRNA with the presence of stem or progenitor cells.
2. The method of claim 1 , wherein the step of determining is performed using a method selected from the group consisting of RT-PCR, in situ hybridization, northern blotting, RNase protection, and any combination of one or more thereof.
3. The method of claim 1 , wherein the stem or progenitor cells are adult stem cells.
4. The method of claim 3 , wherein the adult stem or progenitor cells are selected from the group consisting of mononuclear cells and hematopoietic stem or progenitor cells.
5. The method of claim 4 , wherein the hematopoietic stem or progenitor cells are CD34+ hematopoietic stem or progenitor cells.
6. The method of claim 1 , wherein the tissue, a tissue sample or a cell population is obtained from a mammal.
7. The method of claim 6 , wherein the mammal is selected from the group consisting of primates, rodents, ovine species, bovine species, porcine species, equine species, feline species and canine species.
8. The method of claim 6 , wherein the mammal is a primate.
9. The method of claim 6 , wherein the mammal is a human.
10. The method of claim 1 wherein the stem or progenitor cells are hemangiogenic stem or progenitor cells.
11. The method of claim 10 , wherein the hemangiogenic stem or progenitor cells are hematopoietic stem or progenitor cells.
12. The method of claim 10 , wherein the hemangiogenic stem or progenitor cells are endothelial stem or progenitor cells.