Chiral indole intermediates and their fluorescent cyanine dyes containing functional groups

1. A compound of the following general formula (XIV)

wherein:

R a , R c , R d , and R f are independently selected from V or L-V where

L is a bond or C 1-22 straight or branched alkyl, optionally containing 0, 1 or 2 unsaturations or unsaturated pendent or interchain groups selected from alkenyl, alkynyl and aryl groups;

V is selected from hydrogen, halogen, —OH, —NH 2 , —SH, —CN, trifluoromethyl, —SO 3 − , phosphate, phosphonate, quaternary ammonium, —NO 2 , mono- or di-nitro-substituted benzyl, —COOH, and —NHCOR g , where R g is C 1-20 straight or branched alkyl; a target bonding group, reactive group, reactive moiety, or NHR h where R h is H, C 1-20 straight or branched alkyl or COOH;

T is

wherein each L and V are independently as defined above;

Y is selected from the group consisting of O, S, —CH═CH—, >C(CH 3 ) 2 ,

and —N—(CH 2 ) 1-10 X, wherein X is selected from —COOH, —NH 2 , —SO 3 − , —OH and halogen;

Z a and Z b are independently, fused

and p, u, v and r are independently an integer from 0-4, and at least one V must be —COOH in at least one of T and Y.

2. A compound of claim 1 wherein said reactive group is selected from:

where n is an integer of 1-10, and at least one of Q or W is a leaving group selected from the group consisting of I, Br and Cl.

3. A method of detecting any one of an antibody, protein, peptide, enzyme substrate, hormone, lymphokine, metabolite, receptor, antigen, hapten, lectin, avidin, streptavidin, toxin, carbohydrate, oligosaccharide, polysaccharide, nucleic acid, derivatized deoxy nucleic acid, DNA fragment, RNA fragment, derivatized DNA fragment, derivatized RNA fragment, nucleoside, nucleotide, natural drug, synthetic drug, virus particle, bacterial particle, virus component, yeast component, blood cell, blood cell component, plasma component, serum component, biological cell, noncellular blood component, bacteria, bacterial component, natural or synthetic lipid vesicle, poison, environmental pollutant, polymer, polymer particle, glass particle, glass surface, plastic particle, plastic surface, polymer membrane, conductor or semiconductor comprising detecting a compound of claim 1 bound to said antibody, protein, peptide, enzyme substrate, hormone, lymphokine, metabolite, receptor, antigen, hapten, lectin, avidin, streptavidin, toxin, carbohydrate, oligosaccharide, polysaccharide, nucleic acid, derivatized deoxy nucleic acid, DNA fragment, RNA fragment, derivatized DNA fragment, derivatized RNA fragment, nucleoside, nucleotide, natural drug, synthetic drug, virus particle, bacterial particle, virus component, yeast component, blood cell, blood cell component, plasma component, serum component, biological cell, noncellular blood component, bacteria, bacterial component, natural or synthetic lipid vesicle, poison, environmental pollutant, polymer, polymer particle, glass particle, glass surface, plastic particle, plastic surface, polymer membrane, conductor or semiconductor.

4. A method of detecting any one of an antibody, protein, peptide, enzyme substrate, hormone, lymphokine, metabolite, receptor, antigen, hapten, lectin, avidin, streptavidin, toxin, carbohydrate, oligosaccharide, polysaccharide, nucleic acid, derivatized deoxy nucleic acid, DNA fragment, RNA fragment, derivatized DNA fragment, derivatized RNA fragment, nucleoside, nucleotide, natural drug, synthetic drug, virus particle, bacterial particle, virus component, yeast component, blood cell, blood cell component, plasma component, serum component, biological cell, noncellular blood component, bacteria, bacterial component, natural or synthetic lipid vesicle, poison, environmental pollutant, polymer, polymer particle, glass particle, glass surface, plastic particle, plastic surface, polymer membrane, conductor or semiconductor comprising detecting a compound of claim 2 bound to said antibody, protein, peptide, enzyme substrate, hormone, lymphokine, metabolite, receptor, antigen, hapten, lectin, avidin, streptavidin, toxin, carbohydrate, oligosaccharide, polysaccharide, nucleic acid, derivatized deoxy nucleic acid, DNA fragment, RNA fragment, derivatized DNA fragment, derivatized RNA fragment, nucleoside, nucleotide, natural drug, synthetic drug, virus particle, bacterial particle, virus component, yeast component, blood cell, blood cell component, plasma component, serum component, biological cell, noncellular blood component, bacteria, bacterial component, natural or synthetic lipid vesicle, poison, environmental pollutant, polymer, polymer particle, glass particle, glass surface, plastic particle, plastic surface, polymer membrane, conductor or semiconductor.

5. A method of analysis or detection comprising analyzing or detecting a sample comprising a compound according to claim 1 or a conjugate of a component comprising the same.

6. A method of analysis or detection comprising analyzing or detecting a sample comprising a compound according to claim 2 or a conjugate of a component comprising the same.

7. The method of claim 5 where said detecting is by optical means.

8. The method of claim 6 where said detecting is by optical means.

9. A compound selected from the group consisting of 6-[9-(5-carboxypentyl)-5,7-dimethyl-5,11-disulfo-1,2,9,14,15,16,15a,16a,2a-nonahydroindolo-[2″,1″-1′,2′]isoquinolino[7′,6′-4,3]pyridino[1,2-a]indolin-7-yl/hexanoic acid, and 6,7,9,10-tetrahydro-2,14-disulphonato-16,16,18-trimethyl-7aH,8aH-bisindolinium[3,2-a,3′2′-a]pyrano[3,2-c;5,6-c′]dipyridin-5-ium-18-hexanoic acid.