Nucleic acids and corresponding proteins entitled 202P5A5 useful in treatment and detection of cancer
A novel gene 202P5A5 and its encoded protein, and variants thereof, are described wherein 202P5A5 exhibits tissue specific expression in normal adult tissue, and is aberrantly expressed in the cancers listed in Table I. Consequently, 202P5A5 provides a diagnostic, prognostic, prophylactic and/or therapeutic target for cancer. The 202P5A5 gene or fragment thereof, or its encoded protein, or variants thereof, or a fragment thereof, can be used to elicit a humoral or cellular immune response; antibodies or T cells reactive with 202P5A5 can be used in active or passive immunization.
1. A method for detecting, in a sample, the presence of a 202P5A5 mRNA, comprising the steps of:
producing cDNA from the sample by reverse transcription using at least one primer;
amplifying the cDNA so produced using sense and antisense primers to amplify a 202P5A5 cDNA that encodes the amino acid of SEQ ID NO: 3, wherein the cDNA consists of residues 29-1858 of SEQ ID NO: 2, with or without the stop codon, wherein the nucleotide at each of positions 138 and 1269 is G and the nucleotide at each of positions 1288 and 1662 is A, and the nucleotide at position 1552 is A or G; and
detecting the presence of the amplified 202P5A5 cDNA, thereby detecting the presence of a 202P5A5 mRNA.
2. The method of claim 1 , wherein the sample is taken from a patient who has or who is suspected of having cancer.
3. The method of claim 2 , further comprising:
performing the producing, amplifying and detecting steps on a corresponding normal sample; and
determining whether the 202P5A5 mRNA is elevated, whereby the detection of an elevated 202P5A5 mRNA from the patient tissue sample relative to the normal sample indicates the presence or status of a cancer.
4. The method of claim 3 , wherein the cancer occurs in a tissue selected from among prostate, bladder, colon, lung, ovary, breast, stomach, cervix, lymphoma, bone, and skin.
5. The method of claim 2 , wherein the patient tissue sample is from a tissue selected from among prostate, bladder, colon, lung, ovary, breast, stomach, cervix, lymphoma, bone, and skin.
6. The method of claim 1 , wherein the sample is a biological sample from a patient tissue, the tissue selected from among prostate, bladder, colon, lung, ovary, breast, stomach, cervix, lymphoma, bone, and skin.
7. A method for detecting, in a sample, the presence of a 202P5A5 polynucleotide, comprising steps of:
contacting the sample with a substance that specifically binds to the polynucleotide consisting of residues 29-1858 of SEQ ID NO: 2, with or without the stop codon, wherein the nucleotide at each of positions 138 and 1269 is and the nucleotide at each of positions 1288 and 1662 is A, and the nucleotide at position 1552 is A or G; and
determining that there is a complex of the substance with the polynucleotide in the sample, thereby detecting the presence of the 202P5A5 polynucleotide.
8. The method of claim 7 , wherein the sample is taken from a patient who has or who is suspected of having cancer.
9. The method of claim 7 , wherein the sample is a biological sample from a patient tissue, the tissue selected from among prostate, bladder, colon, lung, ovary, breast, stomach, cervix, lymphoma, bone, and skin.