Chimeric AAV capsid proteins
View Patent ↗Recombinant adeno-associated viral (AAV) capsid proteins are provided. Methods for generating the recombinant adeno-associated viral capsid proteins and a library from which the capsids are selected are also provided.
1. A nucleotide sequence selected from the group of sequences consisting of (i) sequences having at least 95% sequence identity over the entire length of SEQ ID NO:2 and (ii) SEQ ID NO:2.
2. A method of introducing a desired mutation in a target nucleic acid sequence in a mammalian genome of an isolated mammalian cell, comprising:
constructing a recombinant AAV vector comprising a sequence having the desired mutation, wherein said sequence having the desired mutation is encapsidated into a capsid protein, wherein the capsid protein is encoded by a nucleic acid sequence selected from the group of sequences consisting of (i) sequences having at least 95% sequence identity over the entire length of SEQ ID NO:2 and (ii) SEQ ID NO:2; and
introducing said recombinant AAV vector into the isolated mammalian cell.
3. A method of ex vivo gene transfer, comprising:
constructing a recombinant AAV vector comprising a DNA of interest, wherein said DNA of interest is encapsidated into a capsid protein, wherein the capsid protein is encoded by a nucleic acid sequence selected from the group of sequences consisting of (i) sequences having at least 95% sequence identity over the entire length of SEQ ID NO:2 and (ii) SEQ ID NO:2; and
infecting mammalian cells, ex vivo with said recombinant AAV vector comprising the DNA of interest.