Ligand-regulable transactivation systems, methods of use thereof, methods of detecting estrogen receptor ligands, and methods of differentiating estrogen receptor ligand agonists and antagonists
View Patent ↗Briefly described, embodiments of this disclosure include ligand-regulable transactivation systems, methods of producing ligand-regulable transactivation systems, methods of using ligand-regulable transactivation systems, reporter polynucleotides, method of producing reporter polynucleotides, activator fusion proteins, methods of producing activator fusion proteins, methods of regulating gene expression in vitro and in vivo for gene therapy, methods of screening estrogen receptor modulators with therapeutic treatments (e.g., anticancer, antiosteoporosis, and hormone replacement treatments), method of screening compounds (e.g., drugs and environmental pollutants) for the estrogenic effect, methods of evaluating the estrogen receptor pathway under different pathological conditions are provided, and the like.
1. A method of detecting a ligand in vitro, comprising:
providing a ligand-regulable transactivation system comprising:
a reporter polynucleotide that comprises a binding sequence, a promoter sequence, and a reporter sequence encoding a detectable bioluminescent protein, wherein the binding sequence is connected with the promoter sequence and the promoter sequence is connected with the reporter sequence; and
an activator fusion protein that comprises:
a DNA binding domain capable of interacting with the binding sequence of the reporter polynucleotide, an estrogen receptor folding domain (ER folding domain) capable of binding a ligand, wherein the ER folding domain is selected from: a sequence consisting of SEQ ID NO:1 (human estrogen receptor, alpha, amino acids 281-549), a sequence consisting of SEQ ID NO:2 (human estrogen receptor, alpha, amino acids 281-595), a sequence consisting of SEQ ID NO:4 (mouse estrogen receptor, alpha, amino acids 281-549), a sequence consisting of SEQ ID NO:5 (mouse estrogen receptor, alpha, amino acids 281-599), SEQ ID NO:23 and SEQ ID NO:24, and
a transactivation domain capable of interacting with the promoter sequence of the reporter polynucleotide,
wherein the DNA binding domain is connected to the estrogen receptor folding domain, and the estrogen receptor folding domain is connected with the transactivation domain, and wherein interaction of the DNA binding domain and the transactivation domain of the activator fusion protein with the binding sequence and promoter sequence of the reporter polynucleotide regulates expression of the detectable bioluminescent protein;
introducing a ligand to the system; and
detecting a bioluminescent signal in the presence of a bioluminescence initiating compound if the ligand causes the ER folding domain to change from a non-interacting conformational position to an interacting conformational position, wherein the interacting conformational position positions the DNA binding domain and the transactivation domain of the activator fusion protein so that both interact with the binding sequence and the promoter sequence of the reporter polynucleotide, which causes expression of the detectable bioluminescent protein encoded by the reporter sequence.
2. The method of claim 1 , wherein the ligand is selected from: ER ligands, ER agonists, partial ER agonists, ER antagonists, partial ER antagonists, and selective estrogen receptor modulators.
3. The method of claim 1 , wherein the interacting conformation position corresponds to one of two states including substantially interacting and partially interacting, wherein substantially interacting means that the DNA binding domain and the transactivation domain interact with the binding sequence and the promoter sequence of the reporter polynucleotide to generate a first amount of bioluminescent protein, and wherein partially interacting means that the DNA binding domain and the transactivation domain interact with the binding sequence and the promoter sequence of the reporter polynucleotide to generate a second amount of bioluminescent protein, wherein the first amount of bioluminescent protein is greater than the second amount of bioluminescent protein.
4. The method of claim 1 , wherein the reporter sequence is selected from polynucleotide sequences encoding one of the following: a luciferase and a photoprotein.
5. The method of claim 1 , wherein the reporter sequence is selected from polynucleotide sequences encoding one of the following: a Renilla Luciferase; a Coleoptera Luciferase; a Firefly Luciferase; a Gaussia Luciferase; and an aequorin photoprotein Luciferase.
6. The method of claim 1 , wherein the promoter sequence is selected from: an E4 promoter (SEQ ID NO:36), an E4 minimal promoter (SEQ ID NO:18), minimal promoter thymidine kinase (tk-promoter) (SEQ ID NO:18), and an adenoviral late promoter (SEQ ID NO:38).
7. The method of claim 1 , wherein the binding sequence is a GAL4 binding sequence (SEQ ID NO:17).
8. The method of claim 1 , wherein the DNA binding domain is GAL4 DNA binding domain (SEQ ID NO:14).
9. The method of claim 1 , wherein the transactivation domain is VP16 transactivation domain (SEQ ID No: 16).
10. The method of claim 1 , wherein the ligand-regulable transactivation system is expressed in an isolated living cell or culture of cells.
11. A method of detecting a ligand in vivo, comprising:
implanting cells into a nude mouse, wherein the cells comprise exogenous polynucleotides expressing a ligand-regulable transactivation system comprising:
a reporter polynucleotide that comprises a GAL 4 binding sequence, a promoter sequence, and a reporter sequence encoding a detectable bioluminescent protein, wherein the binding sequence is connected with the promoter sequence and the promoter sequence is connected with the reporter sequence; and
an activator fusion protein that comprises:
a DNA binding domain capable of interacting with the binding sequence of the reporter polynucleotide,
an estrogen receptor folding domain (ER folding domain) capable of binding a ligand, wherein the ER folding domain is selected from: SEQ ID NO:23 and SEQ ID NO:24, and
a VP16 transactivation domain capable of interacting with the promoter sequence of the reporter polynucleotide,
wherein the DNA binding domain is connected to the estrogen receptor folding domain, and the estrogen receptor folding domain is connected with the transactivation domain, and wherein interaction of the DNA binding domain and the transactivation domain of the activator fusion protein with the binding sequence and promoter sequence of the reporter polynucleotide regulates expression of the detectable bioluminescent protein;
introducing a ligand to the animal, wherein the ligand is not an endogenous ligand in the mouse; and
detecting a bioluminescent signal in the presence of a bioluminescence initiating compound if the ligand causes the ER folding domain to change from a non-interacting conformational position to an interacting conformational position, wherein the interacting conformational position positions the DNA binding domain and the transactivation domain of the activator fusion protein so that both interact with the binding sequence and the promoter sequence of the reporter polynucleotide, which causes expression of the detectable bioluminescent protein encoded by the reporter sequence.
12. The method of claim 11 , wherein the ligand is selected from: ER ligands, ER agonists, partial ER agonists, ER antagonists, partial ER antagonists, and selective estrogen receptor modulators.
13. The method of claim 11 , wherein the interacting conformation position corresponds to one of two states including substantially interacting and partially interacting, wherein substantially interacting means that the DNA binding domain and the transactivation domain interact with the binding sequence and the promoter sequence of the reporter polynucleotide to generate a first amount of bioluminescent protein, and wherein partially interacting means that the DNA binding domain and the transactivation domain interact with the binding sequence and the promoter sequence of the reporter polynucleotide to generate a second amount of bioluminescent protein, wherein the first amount of bioluminescent protein is greater than the second amount of bioluminescent protein.
14. The method of claim 11 , wherein the reporter sequence is selected from polynucleotide sequences encoding one of the following: a luciferase and a photoprotein.
15. The method of claim 11 , wherein the reporter sequence is selected from polynucleotide sequences encoding one of the following: a Renilla Luciferase; a Coleoptera Luciferase; a Firefly Luciferase; a Gaussia Luciferase; and an aequorin photoprotein Luciferase.
16. The method of claim 11 , wherein the promoter sequence is selected from: an E4 promoter (SEQ ID NO:36), an E4 minimal promoter (SEQ ID NO:18), minimal promoter thymidine kinase (tk-promoter) (SEQ ID NO:18), and an adenoviral late promoter (SEQ ID NO:38).
17. The method of claim 11 , wherein the VP16 transactivation domain has the sequence of SEQ ID NO:16.
18. A method of detecting a ligand in vitro, comprising:
providing a ligand-regulable transactivation system comprising:
a reporter polynucleotide that comprises a GAL4 binding sequence of SEQ ID NO:17, a promoter sequence, and a reporter sequence encoding a detectable bioluminescent protein, wherein the binding sequence is connected with the promoter sequence and the promoter sequence is connected with the reporter sequence; and
an activator fusion protein that comprises:
a DNA binding domain capable of interacting with the GAL4 binding sequence of the reporter polynucleotide,
an estrogen receptor folding domain (ER folding domain) capable of binding a ligand, and
a transactivation domain capable of interacting with the promoter sequence of the reporter polynucleotide,
wherein the DNA binding domain is connected to the estrogen receptor folding domain, and the estrogen receptor folding domain is connected with the transactivation domain, and wherein interaction of the DNA binding domain and the transactivation domain of the activator fusion protein with the binding sequence and promoter sequence of the reporter polynucleotide regulates expression of the detectable bioluminescent protein;
introducing a ligand to the system; and
detecting a bioluminescent signal in the presence of a bioluminescence initiating compound if the ligand causes the ER folding domain to change from a non-interacting conformational position to an interacting conformational position, wherein the interacting conformational position positions the DNA binding domain and the transactivation domain of the activator fusion protein so that both interact with the binding sequence and the promoter sequence of the reporter polynucleotide, which causes expression of the detectable bioluminescent protein encoded by the reporter sequence.
19. The method of claim 18 , wherein the estrogen receptor folding domain is selected from: SEQ ID NO:1 (human estrogen receptor, alpha, amino acids 281-549), SEQ ID NO:2 (human estrogen receptor, alpha, amino acids 281-595), SEQ ID NO:3 (human estrogen receptor, alpha, amino acids 1-595), SEQ ID NO:4 (mouse estrogen receptor, alpha, amino acids 281-549), SEQ ID NO:5 (mouse estrogen receptor, alpha, amino acids 281-599), SEQ ID NO:6 (mouse estrogen receptor amino acids 1-599), SEQ ID NO:23 and SEQ ID NO:24.