Fluorescent sensor based on two fluorescent moieties one of which is a semiconductor nanocrystal and methods of using and making
Binding an analyte can cause a change in fluorescence emission of a sensor. The change in fluorescence can be related to the amount of analyte present. The sensor can include a semiconductor nanocrystal linked to a fluorescent moiety. Upon excitation, the fluorescent moiety can transfer energy to the semiconductor nanocrystal, or vice versa.
1. A composition comprising:
a first fluorescent moiety;
a second fluorescent moiety chemically bound to the first fluorescent moiety;
wherein the first fluorescent moiety is capable of transferring energy to the second fluorescent moiety when excited, or the second fluorescent moiety is capable of transferring energy to the first fluorescent moiety when excited,
the first fluorescent moiety is a semiconductor nanocrystal including a core including a first semiconductor material, one or more optional overcoatings including a second semiconductor material on a surface of the core, and an outer layer coated on the core or, if present, coated on the one or more optional overcoatings, and
wherein the second fluorescent moiety is capable of binding an analyte.
2. The composition of claim 1 , wherein the outer layer includes a compound linked to a surface of the core or, if present, the one or more optional overcoatings, and having a hydrophobic region.
3. The composition of claim 2 , wherein the outer layer further comprises an amphiphilic compound having a hydrophobic region interacting via hydrophobic interactions with the hydrophobic region of the compound linked to a surface of the core or, if present, the one or more optional overcoatings.
4. The composition of claim 3 , wherein the amphiphilic compound is linked to the second fluorescent moiety.
5. The composition of claim 3 , wherein the amphiphilic compound is linked to a plurality of the second fluorescent moieties.
6. The composition of claim 5 , wherein the amphiphilic compound is an amphiphilic polymer.
7. The composition of claim 1 , wherein the semiconductor nanocrystal includes an overcoating including a second semiconductor material on a surface of the core.
8. The composition of claim 7 , wherein the semiconductor nanocrystal includes a second overcoating on a surface of the overcoating including a third semiconductor material.
9. The composition of claim 1 , wherein the outer layer includes an amphiphilic polymer.
10. The composition of claim 1 , wherein the outer layer includes a polyethylene glycol or a dendrimer.
11. The composition of claim 1 , wherein the core includes ZnSe or CdZnS.
12. The composition of claim 1 , wherein the second fluorescent moiety is covalently or noncovalently bound to the first fluorescent moiety.
13. A method of detecting an analyte, comprising:
illuminating a composition with an excitation wavelength, wherein the composition includes:
a first fluorescent moiety;
a second fluorescent moiety chemically bound to the first fluorescent moiety;
wherein the first fluorescent moiety is capable of transferring energy to the second fluorescent moiety when excited, or the second fluorescent moiety is capable of transferring energy to the first fluorescent moiety when excited,
the first fluorescent moiety is a semiconductor nanocrystal including a core including a first semiconductor material, one or more optional overcoatings including a second semiconductor material on a surface of the core, and an outer layer coated on the core or, if present, coated on the one or more optional overcoatings, and
wherein the second fluorescent moiety is capable of binding an analyte; and
measuring a fluorescent emission of the composition.
14. The method of claim 13 , wherein measuring a fluorescent emission of the composition includes measuring a fluorescence emission intensity at an emission wavelength of the first fluorescent moiety.
15. The method of claim 13 , wherein measuring a fluorescent emission of the composition includes measuring a fluorescence emission intensity at an emission wavelength of the second fluorescent moiety.
16. The method of claim 13 , wherein measuring a fluorescent emission of the composition includes measuring a ratio of a fluorescence emission intensity of the first fluorescent moiety to a fluorescence emission intensity of the second fluorescent moiety.
17. The method of claim 16 , further comprising relating the measured ratio to a concentration of the analyte.
18. The method of claim 13 , wherein measuring a fluorescent emission of the composition includes determining a ratio between a fluorescence emission intensity of the first fluorescent moiety to a fluorescence emission intensity of the emissive isosbestic point for the composition or between a fluorescence emission intensity of the second fluorescent moiety to a fluorescence emission intensity of the emissive isosbestic point for the composition.
19. The method of claim 13 , wherein measuring includes performing single-photon excited fluorescence microscopy or multi-photon laser scanning microscopy.
20. A method of making a composition comprising:
linking a first fluorescent moiety to a second fluorescent moiety,
wherein the first fluorescent moiety is a semiconductor nanocrystal including a core including a first semiconductor material, one or more optional overcoatings including a second semiconductor material on a surface of the core, and an outer layer coated on the core or, if present, coated on the one or more optional overcoatings, and
wherein the second fluorescent moiety is capable of binding an analyte.
21. The method of claim 20 , wherein after linking the first fluorescent moiety, when excited, is capable of transferring energy to the second fluorescent moiety, or, when excited, the second fluorescent moiety is capable of transferring energy to the first fluorescent moiety.
22. The method of claim 20 , wherein the outer layer includes a compound linked to a surface of the core or, if present, the one or more optional overcoatings, and having a hydrophobic region.
23. The method of claim 22 , wherein linking includes contacting the semiconductor nanocrystal with an amphiphilic compound having a hydrophobic region.
24. The method of claim 23 , further comprising linking the second fluorescent moiety to the amphiphilic compound.
25. The method of claim 24 , wherein the amphiphilic compound is linked to the second fluorescent moiety.
26. The method of claim 20 , wherein linking includes coupling a surface of the nanocrystal and the second fluorescent moiety with a polyethylene glycol or a dendrimer.
27. The method of claim 20 , wherein linking includes contacting the semiconductor nanocrystal with a compound including a coordinating group having an affinity for a surface of the semiconductor nanocrystal, and a fluorescent moiety.
28. The method of claim 20 , wherein the outer layer includes a compound having a first reactive functional group.
29. The method of claim 28 , wherein linking includes contacting the semiconductor nanocrystal with a compound including a second reactive functional group capable of reacting with the first reactive functional group to form a bond, and a fluorescent moiety.
30. The method of claim 20 , wherein the core includes ZnSe or CdZnS.