Sphingomyelin liposomes for the treatment of hyperactive bladder disorders
The present invention provides pharmaceutical compositions and methods for the instillation of lipid vehicles comprised of liposomes containing sphingomyelin or sphingomyelin metabolites to prevent, manage, ameliorate and/or treat disorders involving neuropathic pain and aberrant muscle contractions, such as what occurs in bladder hyperactivity disorders such as interstitial cystitis (IC) in animals or humans in need thereof. Also provided is a liposome-based delivery of drugs, e.g., antibiotics, pain treatments and anticancer agents, to the bladder, genitourinary tract, gastrointestinal system, pulmonary system and other organs or body systems. In particular, liposome-based delivery of vanilloid compounds, such as resiniferatoxin, capsaicin, or tinyatoxin and toxins, such as botulinum toxin is provided for the treatment of bladder conditions, including pain, inflammation, incontinence and voiding dysfunction.
1. A method of treating a bladder disorder selected from the group consisting of interstitial cystitis, urinary detrusor-sphincter dyssynergia, spastic neurogenic bladder, hyperactive bladder, bladder pain, bladder inflammation, and voiding dysfunction in animals or humans in need thereof, comprising administering to the animal or human by filling the bladder by intravesical instillation a therapeutically effective amount of an aqueous suspension of liposomes having entrapped therein a therapeutically effective amount of a botulinum toxin for the treatment of the bladder disorder.
2. The method according to claim 1 , wherein the therapeutically effective amount of the botulinum toxin ranges from a dosage of about 0.1 mg to about 20 mg per kilogram body weight of the animal or human.
3. The method according to claim 1 , wherein the therapeutically effective amount of the botulinum toxin ranges from a dosage of about 0.5 mg to about 10 mg per kilogram body weight of the animal or human.
4. The method according to claim 1 , wherein the therapeutically effective amount of the botulinum toxin ranges from a dosage of about 1.0 mg to about 5.0 mg per kilogram body weight of the animal or human.
5. The method according to claim 1 , wherein the liposomes are formulated from lipids selected from the group consisting of phospholipids, glycolipids, sphingolipids, sphingophospholipids, and sphingoglycolipds.
6. The method according to claim 5 , wherein the lipids are selected from the group consisting of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidylglycerol, cardiolipin, glycolipids, sphingomyelin, sphingosine 1-phosphate, ceramide galactopyranoside, gangliosides, cerebroside, cholesterol, 1,2-distearoyl-sn-glycero-3-phosphocholine, 1,2-dioleoylphosphatidylcholine and combinations thereof.
7. The method according to claim 6 , wherein the lipids are sphingomyelin.
8. The method according to claim 1 , wherein the voiding dysfunction is incontinence.
9. The method of claim 1 , wherein the botulinum toxin is selected from botulinum toxin A, botulinum toxin B, botulinum toxin C, botulinum toxin D, botulinum toxin E, botulinum toxin F and botulinum toxin G.