Polypeptide having NADH dependent HMF reductase activity
The invention relates to an isolated polypeptide having NADH dependent HMF reductase activity, wherein said polypeptide shows 80% homology to the amino acid sequence shown in SEQ ID NO:2 and which differs from SEQ ID NO:2 in that at least S117L and Y295 or S110 is substituted, a nucleotide sequence coding for said polypeptide, a vector comprising said polypeptide or nucleotide sequence, host comprising said nucleotide sequence or vector as well as the use of the polypeptide for the reduction of furan or carbonyl compounds in lignocellulosic material or in any furan or carbonyl containing material.
1. An isolated polypeptide having NADH dependent HMF reductase activity, wherein said polypeptide shows 90% homology to the amino acid sequence shown in SEQ ID NO:2 and which differs from SEQ ID NO:2 by comprising S117L and also comprising Y295C, Y295S, Y295T or S110P.
2. The isolated polypeptide according to any of claim 1 , wherein said polypeptide differs from SEQ ID NO:2 in that S 117L and Y295C and S110P are substituted.
3. The isolated polypeptide according to claim 1 , wherein said polypeptide has at least 95% homology to SEQ ID NO:2.
4. The isolated polypeptide according to claim 3 , wherein said polypeptide has at least 98% homology to SEQ ID NO:2.
5. The isolated polypeptide according to claim 4 , wherein said polypeptide has at least 98.5% homology to SEQ ID NO:2.
6. The isolated polypeptide according to claim 5 , wherein said polypeptide has at least 98.7% homology to SEQ ID NO:2.
7. The isolated polypeptide according to claim 6 , wherein said polypeptide has at least 98.8% homology to SEQ ID NO:2.
8. The isolated polypeptide according to claim 7 , wherein said polypeptide has at least 99% homology to SEQ ID NO:2.
9. An isolated nucleotide sequence encoding the polypeptide according to claim 1 .
10. A vector comprising a nucleotide sequence as defined in claim 9 .
11. A host cell comprising a nucleotide sequence as defined in claim 10 or a vector as defined in claim 10 .
12. The host cell according to claim 11 , wherein said host cell is selected from the group consisting of eukaryotic and prokaryotic cells.
13. The host cell according to claim 12 , wherein said cells are selected from the group consisting of bacteria, yeast and fungi.
14. The host cell according to claim 13 , wherein said bacteria is selected from the group consisting of Escherichia coli, Klebsiella sp. and Zymomonas mobilis and said fungi is filamentous fungi.
15. The host cell according to claim 13 , wherein said yeast cell is selected from the group Saccharomyces sp., Schizosaccharomyces sp., Kluyveromyces sp., Pichia sp., Hansenula sp., Candida sp., and Yarrowia sp.
16. The host cell according to claim 15 wherein said host cell is selected from the group consisting of Saccharomyces cerevisiae, Saccharomyces bay anus and Saccharomyces carlsbergensis.
17. The host cell according to claim 16 wherein said host cell is Saccharomyces cerevisiae.