Immunotherapy against several tumors including neuronal and brain tumors
The present invention relates to peptides, nucleic acids and cells for use in immunotherapeutic methods. In particular, the present invention relates to the immunotherapy of cancer. The present invention furthermore relates to tumor-associated cytotoxic T cell (CTL) peptide epitopes, alone or in combination with other tumor-associated peptides that serve as active pharmaceutical ingredients of vaccine compositions that stimulate anti-tumor immune responses. The present invention relates to 30 peptide sequences and their variants derived from HLA class I and class II molecules of human tumor cells that can be used in vaccine compositions for eliciting anti-tumor immune responses.
1. A peptide consisting of the amino acid sequence of SEQ ID NO: 4, or a pharmaceutically acceptable salt thereof.
2. A fusion protein comprising the peptide of SEQ ID NO: 4 adjoined at the N-terminus and/or the C-terminus by one or more heterologous peptides, or a pharmaceutically acceptable salt thereof.
3. The fusion protein of claim 2 , wherein said one or more heterologous peptides is a carrier protein selected from keyhole limpet haemocyanin (KLH) and mannan.
4. The fusion protein of claim 2 , wherein the fusion protein maintains an ability to bind to a molecule of the human major histocompatibility complex (MHC) class-I or II, so as to be capable of stimulating CD4+ and/or CD8+ T cells specific.
5. The fusion protein of claim 2 , wherein said fusion protein has an overall length of between 10 and 100 amino acids.
6. The fusion protein of claim 2 , wherein the heterologus peptide comprises the 80 N-terminal amino acids of the HLA-DR antigen-associated invariant chain, Ii.
7. An acylated peptide consisting of SEQ ID NO: 4 or a pharmaceutically acceptable salt thereof.
8. A pegylated peptide consisting of SEQ ID NO: 4 or a pharmaceutically acceptable salt thereof.
9. A composition comprising a peptide of claim 1 or pharmaceutically acceptable salt thereof, and a pharmaceutically a pharmaceutically acceptable carrier.
10. A composition comprising a fusion protein of claim 2 or pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
11. A composition comprising an acylated peptide of claim 7 or pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
12. A composition comprising a pegylated peptide of claim 8 or pharmaceutically acceptable salt thereof, and a pharmaceutically acceptable carrier.
13. A method of inducing an immune response in a subject, the method comprising administering to the subject a peptide of claim 1 or a pharmaceutically acceptable salt thereof.
14. A method of inducing an immune response in a subject, the method comprising administering to the subject a fusion protein of claim 2 or a pharmaceutically acceptable salt thereof.
15. A method of inducing an immune response in a subject, the method comprising administering to the subject an acylated peptide of claim 7 or a pharmaceutically acceptable salt thereof.
16. A method of inducing an immune response in a subject, the method comprising administering to the subject a pegylated peptide of claim 8 or a pharmaceutically acceptable salt thereof.
17. A kit comprising:
(a) container that contains a composition containing, in solution and/or in lyophilized form, a peptide of claim 1 or the fusion protein of claim 2 ;
(b) optionally, a second container comprising a diluent and/or reconstituting solution;
(c) optionally, at least one additional peptide consisting of an amino acid sequence selected from SEQ ID NOs: 1-3 and 5-30; and
(d) optionally, instructions for (i) use of the diluent and/or (ii) reconstitution and/or use of a lyophilized formulation.
18. The kit according to claim 17 , further comprising one or more of (e) a buffer, (f) a diluent, (g) a filter, (h) a needle, and (i) a syringe.