BGL7 beta-glucosidase and nucleic acids encoding the same
The present invention provides BGL7 polypeptides with the biological activity of a β-glucosidase and a method of producing a recombinant enzyme having β-glucosidase activity.
1. A method of producing a polypeptide, comprising:
(a) stably transforming a host cell with an expression vector comprising a nucleic acid sequence which encodes a polypeptide having at least 90% sequence identity to SEQ ID NO:2 wherein said polypeptide has beta-glucosidase 7 (bgl7) activity; and
(b) cultivating said transformed host cell under condition suitable for said host cell to produce said polypeptide.
2. The method of claim 1 wherein the host cell is a filamentous fungi or yeast cell.
3. A cell culture medium comprising a polypeptide that was expressed using recombinant DNA technology, said polypeptide comprising an amino acid sequence having at least 90% identity to the amino acid sequence of SEQ ID NO:2, wherein said polypeptide has beta-glucosidase 7 (bgl7) activity.
4. The cell culture medium of claim 3 , wherein said polypeptide comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ II) NO:2.
5. The cell culture medium of claim 4 , wherein said polypeptide comprises the amino acid sequence of SEQ ID NO:2.
6. The cell culture medium of claim 3 , wherein the polypeptide was secreted from a filamentous fungal cell.
7. The cell culture medium of claim 6 , wherein the filamentous fungal cell is Trichoderma.