Tissue specific promoters
The invention relates to tissue specific promoters which can be used in plants for one or more of the following purposes: a. altering carbohydrate metabolism b. preventing memory substance loss c. expression of an invertase inhibitor d. expression of a fructosyl transferase e. expression of a levan sucrase f. expression of genes coding for transported proteins for N-compounds g. expression of characteristics which increase resistance/tolerance with respect to pathogens.
1. An isolated promoter, which is active in above-ground plant parts, comprising a nucleic acid sequence selected from the group consisting of:
a. SEQ ID NO: 3;
b. the polynucleotide sequence complementary to the full length of SEQ ID NO: 3;
c. a fragment of SEQ ID NO: 3 providing promoter activity in above-ground plant parts; and
d. a fragment of a sequence complementary to the full length of SEQ ID NO: 3, wherein said fragment provides promoter activity in above-ground plant parts.
2. The isolated promoter of claim 1 inserted into a vector or operably connected to a mobile genetic element.
3. A transgenic eukaryotic or a transgenic prokaryotic host cell comprising a promoter, which is active in above-ground plant parts, that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 3 having a promoter activity;
b. the polynucleotide sequence complementary to the full length of SEQ ID NO: 3;
c. a fragment of SEQ ID NO: 3 providing promoter activity in above-ground plant parts; and
d. a fragment of a sequence complementary to the full length of SEQ ID NO: 3, wherein said fragment provides promoter activity in above-ground plant parts.
4. A transgenic plant comprising a promoter that is active in above-ground plant parts and that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 3 having a promoter activity;
b. a sequence complementary to the full length of SEQ ID NO: 3;
c. a fragment of SEQ ID NO: 3 providing promoter activity in above-ground plant parts; and
d. a fragment of a sequence complementary to the full length of SEQ ID NO: 3, wherein said fragment provides promoter activity in above-ground plant parts.
5. The transgenic plant according to claim 4 , wherein said transgenic plant is Beta vulgaris , and wherein said promoter is active in the above-ground plant parts of Beta vulgaris.
6. The transgenic plant according to claim 4 , wherein said transgenic plant is further characterized by the expression of a transgene exclusively in an above-ground plant part, wherein said expression is obtainable by transformation of a plant cell with said promoter that is operably linked to a transgene.
7. A transgenic seed produced by said transgenic plant of claim 4 .
8. The transgenic plant according to claim 6 , wherein said above-ground plant part is a leaf.
9. The transgenic plant according to claim 6 , wherein said plant belongs to species Beta vulgaris , and wherein said promoter is active in the above-ground plant parts of Beta vulgaris.
10. The transgenic plant according to claim 6 , wherein said transgenic plant is characterized by:
a. amending a carbohydrate metabolism;
b. avoiding a loss of a storage substance;
c. expressing an invertase inhibitor;
d. expressing a fructosyl transferase;
e. expressing a levan sucrose;
f. expressing a gene coding for a transporter protein for a N-compound; or
g. developing a feature that increases at least one of resistance and tolerance towards pathogens.
11. A method of:
a. amending carbohydrate metabolism,
b. avoiding loss of storage substance,
c. increasing at least one of resistance and tolerance towards a pathogen, or
d. expressing from a transgene:
an invertase inhibitor,
a fructosyl transferase,
a levan sucrose, or
a transporter protein for a N-compound, in a transgenic plant, said method comprising:
(1) transforming a plant cell with a promoter, which is active in above-ground plant parts, wherein said promoter is operably linked to said transgene, and
(2) subsequently regenerating a transgenic plant from said plant cell,
wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
i. SEQ ID NO: 3;
ii. the polynucleotide sequence complementary to the full length of SEQ ID NO: 3;
iii. a fragment of SEQ ID NO: 3 providing promoter activity in above-ground plant parts; and
iv. a fragment of a sequence complementary to the full length of SEQ ID NO: 3, wherein said fragment provides promoter activity in above-ground plant parts
12. An isolated promoter comprising a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 3; and
b. the polynucleotide sequence complementary to the full length of SEQ ID NO: 3.
13. The isolated promoter of claim 12 inserted into a vector or operably connected to a mobile genetic element.
14. A transgenic eukaryotic or a transgenic prokaryotic host cell comprising a promoter that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 3 having a promoter activity; and
b. the polynucleotide sequence complementary to the full length of SEQ ID NO: 3.
15. A transgenic plant comprising a promoter that is operably linked to a transgene, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
a. SEQ ID NO: 3 having a promoter activity; and
b. the polynucleotide sequence complementary to the full length of SEQ ID NO:3.
16. The transgenic plant according to claim 15 wherein said transgenic plant is Beta vulgaris , and wherein said promoter is active in the above-ground plant parts of Beta vulgaris.
17. The transgenic plant according to claim 15 , wherein said transgenic plant is further characterized by the expression of a transgene exclusively in an above-ground plant part, wherein said expression is obtainable by transformation of a plant cell with said promoter that is operably linked to a transgene.
18. A transgenic seed produced by said transgenic plant of claim 15 .
19. The transgenic plant according to claim 17 , wherein said above-ground plant part is a leaf.
20. The transgenic plant according to claim 17 , wherein said plant belongs to species Beta vulgaris , and wherein said promoter is active in the above-ground plant parts of Beta vulgaris.
21. The transgenic plant according to claim 17 , wherein said transgenic plant is characterized by:
a. amending a carbohydrate metabolism;
b. avoiding a loss of a storage substance;
c. expressing an invertase inhibitor;
d. expressing a fructosyl transferase;
e. expressing a levan sucrose;
f. expressing a gene coding for a transporter protein for an N-compound; or
g. developing a feature that increases at least one of resistance and tolerance towards pathogens.
22. A method of:
a. amending carbohydrate metabolism;
b. avoiding loss of storage substance;
c. increasing at least one of resistance and tolerance towards a pathogen; or
d. expressing from a transgene:
an invertase inhibitor,
a fructosyl transferase,
a levan sucrose, or
a transporter protein from an N-compound, in a transgenic plant, said method comprising:
(1) transforming a plant cell with a promoter, wherein said promoter is operably linked to said transgene; and
(2) subsequently regenerating a transgenic plant from said plant cell, wherein said promoter comprises a nucleotide sequence selected from the group consisting of:
i. SEQ ID NO: 3; and
ii. the polynucleotide sequence complementary to SEQ ID NO: 3.