MCP-1 binding nucleic acids
The present invention is related to a nucleic acid, preferably binding to MCP-1, selected from the group comprising type 1A nucleic acids, type 1B nucleic acids, type 2 nucleic acids, type 3 nucleic acids, type 4 nucleic acids and nucleic acids having a nucleic acid sequence according to any of SEQ. ID. No. 87 to 115.
1. An L-nucleic acid that binds a chemokine, or a homolog of said L-nucleic acid, wherein said L-nucleic acid has the sequence of SEQ ID NO:37, and the homolog has homology to the sequence of SEQ ID NO:37 of at least 85%.
2. The L-nucleic acid of claim 1 , wherein said chemokine comprises MCP-1.
3. The L-nucleic acid of claim 1 , comprising a modification.
4. The L-nucleic acid of claim 3 , wherein said modification comprises a polyethylene glycol (PEG) molecule.
5. The L-nucleic acid of claim 4 , wherein said PEG is at the 5′ terminus of said L-nucleic acid or said homolog.
6. The L-nucleic acid of claim 4 , wherein said PEG is at the 3′ terminus of said L-nucleic acid or said homolog.
7. The L-nucleic acid of claim 4 , wherein said PEG is straight chain or branched.
8. The L-nucleic acid of claim 4 , wherein said PEG is from about 2 kD to about 200 kD.
9. The L-nucleic acid of claim 4 , wherein said PEG is from about 40 kD to about 120 kD.
10. The L-nucleic acid of claim 3 , further comprising a linker.
11. The L-nucleic acid of claim 3 , wherein said modification comprises a biotin.
12. The L-nucleic acid of claim 1 , further comprising a chemokine.
13. The L-nucleic acid of claim 12 , wherein the chemokine is selected from the group consisting of eotaxin, MCP-1 and MCP-2.
14. A method for detecting a chemokine comprising exposing a sample suspected of comprising a chemokine to the L-nucleic acid according to claim 1 and detecting a complex of said L-nucleic acid and said chemokine.
15. The method of claim 14 , wherein the chemokine is selected from the group consisting of eotaxin, MCP-1 and MCP-2.
16. The method of claim 14 , further comprising a reagent immobilized on a solid phase.