Transcriptional engineering of
The invention relates to global transcription machinery engineering to produce altered cells having improved phenotypes and methods for evaluating phenotypic diversity.
1. A mutant sigma factor comprising a nonsynonymous substitution of glutamine 345 (Q345) in Lactobacillus plantarum RpoD (SEQ ID NO:11), or the equivalent amino acid in other sigma factors of Lactobacillus or of other bacterial strains, wherein the sigma factor does not comprise a truncation.
2. The mutant sigma factor of claim 1 , wherein the sigma factor is rpoD, rpoF, rpoS, rpoH, rpoN, rpoE, fed or sigH.
3. The sigma factor of claim 2 , wherein the sigma factor is rpoD.
4. The sigma factor of claim 1 , wherein the bacterial strain is an E. coli strain.
5. The mutant Lactobacillus sigma factor of claim 1 , wherein the nonsynonymous substitution is a lysine (Q345K) in rpoD.