Regulation of mink in thymocytes and T lymphocytes
The invention relates to compositions and methods used to assess and alter the expression and activity of MINK in cells of the immune system, particularly thymocytes and T lymphocytes. The methods and compositions are used in a variety of clinical applications including vaccination, treatment of cancer, infectious disease, allergy, and transplantation. Screening methods are provided to identify inhibitors of MINK and susceptibility to effects of under- or over-expression of MINK.
1. A method for inhibiting negative selection of thymocytes in a mammal, the method comprising down-regulating MINK in thymocytes in a mammal prior to negative selection of said thymocytes, thereby inhibiting negative selection in thymocytes in a mammal.
2. A method for inhibiting activation-induced cell death of mammalian T cells, the method comprising inhibiting activation-induced cell death of mammalian T cells that are repeatedly stimulated by an antigen by down-regulating MINK in mammalian T cells specific for the antigen.
3. The method of claim 2 , wherein the antigen is a microbial antigen.
4. A method for preparing bone marrow cells for use as a bone marrow autograft in a subject in need thereof, the method comprising down-regulating MINK ex vivo in bone marrow cells of the subject, thereby producing a bone marrow autograft for use in said subject in need thereof.