Modified factor VIII and factor IX genes and vectors for gene therapy
The present invention relates to a modified and optimized Factor VIII or Factor IX nucleic acid for inclusion in a chimeric virus vector. Use of such vector can be used for treatment of hemophilia.
1. An isolated optimized gene modified to increase CG sequences and reduce cis motifs relative to a wild type FVIII or wild type FIX gene, wherein the optimized gene is selected from the group consisting of FVIII and FIX, wherein when the optimized gene is FVIII the gene has the nucleotide sequence of SEQ ID NO: 12 or 15 or a nucleotide sequence having 95% identity thereto, and wherein when the optimized gene is FIX the gene has the nucleotide sequence of SEQ ID NO 7 or a nucleotide sequence having 95% identity thereto.
2. The optimized gene of claim 1 , where the CG sequences are increased at least 40% relative to the wild type gene.
3. The optimized gene of claim 1 , included in a virus vector or a chimeric virus vector.
4. The optimized gene of claim 3 , wherein the chimeric virus vector comprises capsid components selected from AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7 AAV8, AAV9, AAV10, AAV11 and AAV12 capsids.
5. The optimized gene of claim 4 , wherein the chimeric virus vector comprises SEQ ID NO: 17 or its complement.
6. The optimized gene of claim 1 , wherein the optimized gene is flanked by AAV TRs.
7. The optimized gene of claim 3 , wherein the virus vector comprises the nucleotide sequence of SEQ ID NO. 1.
8. A method of increasing expression of FVIII or FIX genes in a subject, the method comprising:
providing at least one recombinant virus vector comprising a nucleotide sequences encoding an optimized FVIII or FIX gene according to claim 1 , wherein the optimized genes have been modified to increase CG sequences and reduce cis motifs relative to a wild type FVIII gene or wild type FIX gene, respectively; and
administering the recombinant virus vector to the subject under conditions such that the optimized FVIII or FIX nucleotide sequences are expressed at a level which produces an increased amount of FVIII or FIX in the subject.
9. The method of claim 8 , where the CG sequences are increased at least 40% relative to the wild type gene.
10. The method of claim 8 , wherein the recombinant virus vector is a chimeric virus vector.
11. The method of claim 10 , wherein the chimeric virus vector comprises capsid components selected from AAV1, AAV2, AAV3, AAV4, AAV5, AAV6, AAV7 AAV8, AAV9, AAV10, AAV11 and AAV12 capsids.
12. The method of claim 11 , wherein the chimeric virus vector comprises SEQ ID NO: 17 or its complement.
13. The method of claim 8 , wherein the optimized gene is flanked by AAV TRs.
14. The method of claim 10 , wherein the recombinant virus vector comprises the nucleotide sequence of SEQ ID NO. 1.
15. An in vitro process of expressing an optimized FVIII or FIX peptide comprising; transfecting a cell with polynucleotide that encodes the optimized FVIII or FIX peptide according to claim 1 , to produce a transformed host cell, wherein the polynucleotide encoding the optimized FVIII or FIX genes have been modified to increase CG sequences and reduce cis motifs relative to a wild type FVIII gene or wild type FIX gene; and maintaining the transformed host cell under biological conditions sufficient for expression of the peptide.
16. A composition comprising an admixture of:
an isolated or purified nucleic acid according to claim 1 , and
a carrier.