Pathogen detection biosensor
The invention described herein provides methods for the detection of target particles, such as pathogens, soluble antigens, nucleic acids, toxins, chemicals, plant pathogens, blood borne pathogens, bacteria, viruses and the like. Also described is an emittor cell comprising a receptor, wherein the receptor can be an antibody or an Fc receptor, and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more receptors on the emittor cell. Also provided are optoelectronic sensor devices for detecting a target particle in a sample, including in a plurality of samples.
1. A method for testing for the presence of a plant pathogen in a plant sample comprising:
a) preparing a plant sample for testing for the presence of a plant pathogen;
b) adding beads to the plant sample, wherein the beads bind to a plant pathogen;
c) adding emitter cells to said beads, the emitter cells comprising receptors which are expressed on the surface of the emitter cells and are specific to the plant pathogen to be detected, wherein binding of the plant pathogen to the receptors result in an increase in calcium concentration in the cytosol of the emitter cell, wherein the emitter cells further comprise an emitter molecule that emits a photon in response to the increase in intracellular calcium; and
d) detecting photon emission, wherein photon emission is indicative of a plant pathogen in the sample.
2. The method of claim 1 , wherein the plant pathogen is a virus, fungus or bacteria.
3. The method of claim 1 , wherein the beads are polystyrene beads.
4. The method of claim 3 , wherein the polystyrene beads are magnetic polystyrene beads.
5. The method of claim 4 , wherein the virus is a potyvirus.
6. The method of claim 1 , wherein the beads are coated with a plant pathogen-specific antibody.
7. The method of claim 6 , wherein the beads are magnetic polystyrene beads.
8. The method of claim 6 , wherein the plant pathogen is Phytophthora spp.
9. The method of claim 1 , wherein the emitter molecule comprises a calcium-sensitive luminescent molecule or a calcium-sensitive fluorescent molecule.
10. The method of claim 9 , wherein the emitter molecule is selected from the group consisting of aequorin, obelin, thalassicolin, mitrocomin (halistaurin), clytin (phialidin), mnemiopsin, berovin, Indo-1, Fura-2, Quin-2, Fluo-3, Rhod-2, calcium green, BAPTA, a cameleon and combinations thereof.
11. The method of claim 1 , wherein the emitter cell is selected from the group consisting of a B-cell, a T-cell, a macrophage cell, a mast cell and a fibroblast.
12. The method of claim 1 , wherein the receptor is an antibody.
13. The method of claim 12 , wherein the antibody is an anti-immunoglobulin antibody.
14. The method of claim 1 , wherein the receptor is an Fc receptor.