Method of producing virus
View Patent ↗It is intended to provide a safe and efficient method of producing a virus which is free from animal-origin components in the whole process from culturing adhesive cells to producing the virus on an industrial scale by the cell culture. Namely, a method of producing a virus comprising: adhering adhesive cells to a support which has a polypeptide (P) having at least one cell-adhesive minimum amino acid sequence (X) per molecule, and is free from animal-origin components; culturing the adhesive cells in a medium free from animal-origin components; subculturing the cultured adhesive cells using a cell dispersing agent: free from animal-origin components; and then inoculating and proliferating a virus in the cells obtained by culturing the adhesive cells.
1. A method of producing a virus comprising:
adhering adhesive cells to a support which has a polypeptide of about 20,000 Mn having a structure where 5 (Arg Gly Asp) sequences (SEQ ID NO: 70) and 5 (Gly Ala Gly Ala Gly Ser) 3 sequences (SEQ ID NO: 74) are alternately chemically bonded, and is free from animal-origin components;
culturing the adhesive cells in a medium free from animal-origin components;
subculturing the cultured adhesive cells using a cell dispersing agent that is free from animal-origin components and is a protease originated from a plant, a protease originated from genetically recombinant bacteria, or a combination thereof; and then
inoculating and proliferating a virus in the cells obtained by culturing the adhesive cells, thereby improving efficiency for producing a virus.
2. The method according to claim 1 , wherein said virus belongs to at least one selected from a group consisting of Flaviviridae, Orthomyxoviridae, Adenoviridae, Herpesviridae, Picornaviridae, Paramyxoviridae, Togaviridae, and Poxviridae.
3. The method according to claim 1 or 2 , wherein said support is a microcarrier.
4. A method of producing a virus comprising:
adhering adhesive cells to a support which has a polypeptide of about 10,000 Mn having a structure where 3 (Arg Gly Asp) sequences (SEQ ID NO: 70) and 3 (Gly Val Pro Gly Val) 2 Gly Gly (Gly Ala Gly Ala Gly Ser) 3 sequences (SEQ ID NO: 71) are alternately chemically bonded, and is free from animal-origin components;
culturing the adhesive cells in a medium free from animal-origin components;
subculturing the cultured adhesive cells using a cell dispersing agent that is free from animal-origin components and is a protease originated from a plant, a protease originated from genetically recombinant bacteria, or a combination thereof; and then
inoculating and proliferating a virus in the cells obtained by culturing the adhesive cells, thereby improving efficiency for producing a virus.
5. The method according to claim 4 , wherein said virus belongs to at least one selected from a group consisting of Flaviviridae, Orthomyxoviridae, Adenoviridae, Herpesviridae, Picornaviridae, Paramyxoviridae, Togaviridae, and Poxviridae.
6. The method according to claim 4 or 5 , wherein said support is a microcarrier.