Compositions comprising HMW-MAA and fragments thereof, and methods of use thereof
View Patent ↗This invention provides recombinant polypeptides comprising a fragment of a High Molecular Weight Melanoma-Associated Antigen (HMW-MAA), recombinant Listeria strains comprising same, and methods of inducing an immune response and treating and impeding the growth of tumors, comprising administering same.
1. A recombinant Listeria strain comprising a recombinant nucleotide sequence encoding a recombinant polypeptide, said recombinant polypeptide comprising a fragment of a High Molecular Weight Melanoma-Associated Antigen (HMW-MAA) protein fused to a listeriolysin (LLO) oligopeptide, an ActA oligopeptide, or a PEST oligopeptide, wherein said nucleotide sequence encoding said fragment is selected from the group consisting of SEQ ID NOs: 21, 22 or 23.
2. The recombinant Listeria strain of claim 1 , wherein said HMW-MAA protein is a human HMW-MAA protein.
3. An immunogenic composition comprising the recombinant Listeria strain of claim 1 , or claims 5 - 7 .
4. The immunogenic composition of claim 3 , further comprising an adjuvant, cytokine, chemokine, or combination thereof.
5. A recombinant Listeria strain comprising a recombinant nucleotide sequence encoding a recombinant polypeptide, said recombinant polypeptide comprising a fragment of a High Molecular Weight Melanoma-Associated Antigen (HMW-MAA) protein fused to a listeriolysin (LLO) oligopeptide, an ActA oligopeptide, or a PEST oligopeptide, wherein said nucleotide sequence encodes SEQ ID NO: 21.
6. A recombinant Listeria strain comprising a recombinant nucleotide sequence encoding a recombinant polypeptide, said recombinant polypeptide comprising a fragment of a High Molecular Weight Melanoma-Associated Antigen (HMW-MAA) protein fused to a listeriolysin (LLO) oligopeptide, an ActA oligopeptide, or a PEST oligopeptide, wherein said nucleotide sequence encodes SEQ ID NO: 22.
7. A recombinant Listeria strain comprising a recombinant nucleotide sequence encoding a recombinant polypeptide, said recombinant polypeptide comprising a fragment of a High Molecular Weight Melanoma-Associated Antigen (HMW-MAA) protein fused to a listeriolysin (LLO) oligopeptide, an ActA oligopeptide, or a PEST oligopeptide, wherein said nucleotide sequence encodes SEQ ID NO: 23.
8. The recombinant Listeria strain of claim 1 , wherein said recombinant Listeria strain is a recombinant Listeria monocytogenes strain.
9. The recombinant Listeria strain of claim 1 , wherein said recombinant Listeria strain has been passaged through an animal host.