Chimeric NK receptor and methods for treating cancer
The present invention relates to chimeric immune receptor molecules for reducing or eliminating tumors. The chimeric receptors are composed a C-type lectin-like natural killer cell receptor, or a protein associated therewith, fused to an immune signaling receptor containing an immunoreceptor tyrosine-based activation motif. Methods for using the chimeric receptors are further provided.
1. A nucleic acid construct for expressing a chimeric polypeptide receptor to reduce or eliminate a tumor comprising:
a first nucleic acid sequence encoding a promoter operably linked to
a second nucleic acid sequence encoding a chimeric polypeptide receptor comprising DAP10 fused to an immune signaling receptor containing an immunoreceptor tyrosine-based activation motif of SEQ ID NO: 1.
2. A vector comprising the nucleic acid construct of claim 1 .
3. The nucleic acid construct of claim 1 further comprising a suicide gene.
4. An isolated T cell comprising the nucleic acid construct of claim 1 or claim 3 .
5. An isolated T cell comprising the vector of claim 2 .