Intelligent nanomedicine integrating diagnosis and therapy
View Patent ↗A method of controlling the activity of a biologically active compound. The method concerns an oligonucleotide-based compound, comprising a hairpin-forming oligonucleotide, an effector moiety physically associated with the oligonucleotide, where the effector moiety possesses a biological activity, and a regulating moiety physically associated with the oligonucleotide, where the regulating moiety controls the biological activity of the effector moiety by interacting with the effector moiety. The oligonucleotide can assume a hairpin configuration, where the effector and regulating moieties interact, or an open configuration, where the effector and regulating moieties fail to interact. Depending on the nature of the effector and regulating moieties, either configuration can result in the expression of the biological activity of the effector moiety.
1. A method of controlling the activity of a biologically active compound, comprising:
a) providing a hairpin-forming oligonucleotide capable of existing in either a hairpin configuration having a single-stranded loop and a double-stranded stem, or an open configuration lacking the double-stranded stem;
b) physically associating an effector moiety with the oligonucleotide, said effector moiety having a biological activity;
c) physically associating a regulating moiety with the oligonucleotide, said regulating moiety controlling the biological activity of the effector moiety when the regulating and effector moieties interact; and
d) changing from one configuration to the other;
wherein the regulating moiety interacts with the effector moiety when the oligonucleotide is in the hairpin configuration, and the regulating and effector moieties fail to interact when the oligonucleotide is in the open configuration, and
wherein the biological activity comprises sterically blocking a target molecule.
2. The method of claim 1 , wherein the biological activity is a therapeutic activity.
3. The method of claim 1 , wherein the regulating moiety activates the biological activity of the effector moiety.
4. The method of claim 1 , wherein the regulating moiety inhibits the biological activity of the effector moiety.
5. The method of claim 1 , wherein binding of the loop to a biomarker causes the oligonucleotide to assume its open configuration, whereby the biological activity of the effector moiety is expressed.
6. The method of claim 5 , wherein the biomarker is a protein or a nucleic acid.
7. The method of claim 1 , further comprising providing a binding partner that binds to the loop, thereby causing the oligonucleotide to assume its open configuration.
8. The method of claim 7 , wherein the binding partner is a protein or a nucleic acid.
9. The method of claim 7 , wherein binding of a biomarker to the binding partner causes the oligonucleotide to assume its hairpin configuration, whereby the biological activity of the effector moiety is expressed.
10. The method of claim 1 , wherein the effector moiety comprises an enzyme and the regulating moiety inhibits the enzyme when the oligonucleotide is in the hairpin configuration.
11. The method of claim 1 , wherein the effector moiety comprises a prodrug and the regulating moiety converts the prodrug into a therapeutically active agent when the oligonucleotide is in the hairpin configuration.
12. The method of claim 1 , wherein the effector moiety comprises an antisense molecule making up at least a portion of one strand of the duplex stem, and the regulating moiety comprises at least a portion of the other strand of the duplex stem and is complementary to the antisense molecule.
13. The method of claim 1 , wherein the effector moiety comprises a nanofabricated container enclosing molecules having at least one biological activity, and the regulating moiety prevents the release of the molecules from the container when the oligonucleotide is in the hairpin configuration.
14. A method of controlling the activity of a biologically active compound, comprising:
a) providing a hairpin-forming oligonucleotide capable of existing in either a hairpin configuration having a single-stranded loop and a double-stranded stem, or an open configuration lacking the double-stranded stem;
b) attaching an effector moiety to the oligonucleotide, said effector moiety having a therapeutic activity;
c) attaching a regulating moiety to the oligonucleotide, said regulating moiety inhibiting or activating the therapeutic activity of the effector moiety when the regulating and effector moieties interact; and
d) changing from one configuration to the other;
wherein the regulating moiety interacts with the effector moiety when the oligonucleotide is in the hairpin configuration, and the regulating and effector moieties fail to interact when the oligonucleotide is in the open configuration, and
wherein the therapeutic activity comprises sterically blocking a target molecule.
15. The method of claim 14 , wherein binding of the loop to a biomarker causes the oligonucleotide to assume its open configuration, whereby the therapeutic activity of the effector moiety is expressed.
16. The method of claim 15 , wherein the effector moiety is an enzyme and the regulating moiety inhibits the enzyme when the oligonucleotide is in the hairpin configuration.
17. The method of claim 14 , further comprising providing a binding partner that binds to the loop, thereby causing the oligonucleotide to assume its open configuration.
18. The method of claim 17 , wherein binding of a biomarker to the binding partner causes the oligonucleotide to assume its hairpin configuration, whereby the therapeutic activity of the effector moiety is expressed.
19. The method of claim 18 , wherein the effector moiety comprises a prodrug and the regulating moiety converts the prodrug into a therapeutically active agent when the oligonucleotide is in the hairpin configuration.