Modified cells expressing a protein that modulates activity of bHLH proteins, and uses thereof
View Patent ↗The present invention relates to modified cells carrying a heterologous gene sequence encoding a protein, such as an Inhibitor of differentiation (Id) gene sequence that binds a basic helix-loop-helix (bHLH) protein to inhibit cell growth, differentiation and/or tumorigenesis of the modified cells. The modified cells are differentiated, proliferate and do not become tumorigenic when grafted into a recipient subject. Additionally, the modified cells produce a factor or factors that enhance the viability of co-grafted organs, tissues or cells. Thus, the modified cells are useful for testing agents for effects on the cells, for co-grafting with transplant organs, tissues or cells. The modified cells are also useful for enhancing the viability of thawing cells that have been cryo-preserved. In one embodiment, the modified cells are modified Sertoli cells.
1. An isolated modified mammalian cell comprising a stably integrated introduced gene sequence, which stably expresses a protein which binds a basic helix-loop-helix (bHLH) protein, wherein the stably integrated introduced gene sequence is integrated into the cell genome, wherein the cell is a proliferating non-tumorigenic Sertoli cell and was post-mitotic prior to stable integration of the introduced gene sequence into the cell genome, wherein the protein is an Inhibitor of differentiation (Id) protein.
2. The modified cell of claim 1 , which is selected from the group consisting of a differentiating cell and differentiated cell.
3. The modified cell of claim 1 , wherein the Id protein is Id1, Id2, Id3, or Id4.