Fluorescence meter
Disclosed is a fluorescence meter for analyzing a sample, comprising a main beam path and at least one optical module. The fluorescence meter is embodied so as to provide at least one electromagnetic beam for exciting the sample and receive at least one electromagnetic beam emitted by the sample, at least some sections of at least two of the electromagnetic beams being located on one plane while extending along the main beam path.
1. Fluorescence meter for investigating a specimen, comprising:
a housing including a main channel for a main optical path;
a first source for providing an output comprising a first excitation electromagnetic beam disposed along the main optical path to excite the specimen;
a monitor diode disposed near said first source to monitor the output of said first source for deviation from a desired value such that the output can be stabilized;
a first sensor to detect an emitted electromagnetic beam from the specimen having a first spectral range;
a first optical module to direct the emitted electromagnetic beam to said first sensor;
said first optical module is configured such that the emission electromagnetic beam is disposed with the excitation electromagnetic beam at least partly in one plane and extending along the main optical path;
a first band-pass filter disposed between said first source and the specimen;
a second band-pass filter disposed between said first source and said monitor diode, said second band-pass filter not forming a part of an optical path between said first source and the specimen, an optical path between said first source and said monitor diode not including a beam splitter; and
said first and second band-pass filters have the same optical properties such that the electromagnetic beam shining on the specimen and on said monitor diode is the same, whereby the output can be monitored and said first source can be stabilized.
2. Fluorescence meter according to claim 1 , and further comprising:
a second sensor to detect an emitted electromagnetic beam from the specimen having a second spectral range; and
a second optical module to direct the emitted electromagnetic beam having the second spectral range to said second sensor.
3. Fluorescence meter according to claim 2 , wherein said first sensor is configured to detect reflection.
4. Fluorescence meter according to claim 2 , wherein:
said housing includes a first secondary channel branching from said main channel, said first secondary channel for providing a first secondary optical path;
an excitation optical module including a first beam splitter;
said first source is disposed in said first secondary channel; and
said first beam splitter is disposed in said main channel to deflect the first excitation electromagnetic beam into the main optical path.
5. Fluorescence meter according to claim 4 , wherein:
said housing includes a second secondary channel branching from said main channel, said second secondary channel for providing a second secondary optical path;
said first sensor is disposed in said second secondary channel;
said first optical module includes a second beam splitter; and
said second beam splitter is disposed in said main channel to deflect the emission excitation electromagnetic beam having a first spectral range from the main optical path into the second secondary optical path.
6. Fluorescence meter according to claim 5 , wherein:
said housing includes a third secondary channel branching from said main channel, said third secondary channel for providing a third secondary optical path;
said second sensor is disposed in said third secondary channel;
said second optical module includes a third beam splitter; and
said third beam splitter is disposed in said main channel to deflect the emission excitation electromagnetic beam having a second spectral range from the main optical path into the third secondary optical path.
7. Fluorescence meter according to claim 6 , wherein said first, second and third beam splitters are dichroic mirrors.
8. A fluorescence meter according to claim 4 , and further comprising a light trap disposed along said first secondary optical path beyond said first beam splitter.
9. Fluorescence meter according to claim 2 , and further comprising a second source for providing another output comprising a second excitation electromagnetic beam disposed along the main optical path to excite the specimen.
10. Fluorescence meter according to claim 9 , wherein:
said first sensor is configured to detect a first fluorescence emission from the specimen; and
said second sensor is configured to detect a second fluorescence emission from the specimen.
11. A fluorescence meter according to claim 9 , wherein:
the first excitation electromagnetic beam is a measuring beam; and
the second excitation electromagnetic beam is a positioning beam to position the measuring beam from the specimen.
12. A fluorescence meter according to claim 2 , and further comprising:
a second source for providing another output comprising a second excitation electromagnetic beam disposed along the main optical path to excite the specimen;
said housing includes a secondary channel branching from said main channel, said secondary channel for providing a secondary optical path;
an excitation optical module including a first beam splitter;
said second source is disposed in said secondary channel;
said first beam splitter is disposed in said main channel to deflect the second excitation electromagnetic beam into the main optical path; and
a light trap disposed along said secondary optical path beyond said first beam splitter.
13. Fluorescence meter according to claim 1 , and further comprising a marking within said housing for an eye to concentrate on during examination.
14. Fluorescence meter according to claim 13 , and further comprising an illumination source to provide a background illumination for said marking.
15. Fluorescence meter according to claim 1 , wherein said first source is encapsulated with an encapsulation.
16. Fluorescence meter according to claim 15 , wherein said encapsulation is an aluminum block.
17. Fluorescence meter according to claim 15 , wherein said encapsulation has an opening.
18. Fluorescence meter according to claim 1 , wherein said monitor diode is disposed within said housing to prevent light entering said housing from the outside through said main channel from hitting said monitor diode.
19. Fluorescence meter according to claim 1 , and further comprising an adaptor for taking surface measurements from a dish, test strip, microscope slide or PCR tube.
20. Fluorescence meter for investigating a specimen, comprising:
a housing including a main channel for a main optical path;
a first source for providing an output comprising a first excitation electromagnetic beam disposed along the main optical path to excite the specimen;
a monitor diode disposed near said first source to monitor the output of said first source for deviation from a desired value such that the output can be stabilized;
a band-pass filter disposed between said first source and said monitor diode, said band-pass filter not forming a part of an optical path between said first source and the specimen, an optical path between said first source and said monitor diode not including a beam splitter;
a second source separate from said first source, said second source for providing another output comprising a second excitation electromagnetic beam disposed along the main optical path to excite the specimen;
a first sensor to detect an emitted electromagnetic beam having a first spectral range from the specimen;
a second sensor to detect an emitted electromagnetic beam having a second spectral range from the specimen;
a first optical module to direct the emitted electromagnetic beam having the first spectral range to said first sensor;
a second optical module to direct the emitted electromagnetic beam having the second spectral range to said second sensor; and
said first and second optical modules are configured such that the emission electromagnetic beams are disposed with the excitation electromagnetic beams at least partly in one plane and extending along the main optical path.
21. Fluorescence meter for investigating a specimen, comprising:
a housing including a main channel for a main optical path;
a first source for providing an output comprising a first excitation electromagnetic beam disposed along the main optical path to excite the specimen;
a monitor diode disposed near said first source to monitor the output of said first source for spectral drift such that a change in output caused by the spectral drift can be adjusted;
a first sensor to detect an emitted electromagnetic beam from the specimen having a first spectral range;
a first optical module to direct the emitted electromagnetic beam to said first sensor;
said first optical module is configured such that the emission electromagnetic beam is disposed with the excitation electromagnetic beam at least partly in one plane and extending along the main optical path;
a first band-pass filter disposed in front of said first source;
a second band-pass filter disposed between said first source and said monitor diode, said second band-pass filter not forming a part of an optical path between said first source and the specimen, an optical path between said first source and said monitor diode not including a beam splitter; and
said first and second band-pass filters have the same optical properties such that the electromagnetic beam shining on the specimen and on said monitor diode is the same, whereby the output can be monitored and said first source can be adjusted.