Hydrolytic enzyme mixtures for saccharification of lignocellulosic polysaccharides
The present invention relates to cell wall degradative systems, in particular to systems containing enzymes that bind to and/or depolymerize cellulose. These systems have a number of applications. Some embodiments relate to a method of producing ethanol using the cell wall degradative systems of the present invention.
1. A method for producing saccharides from lignocellulosic material, comprising:
providing lignocellulosic material;
providing a microorganism producing a degradative enzyme mixture derived from Saccharophagus degradans 2-40;
lysing the microorganism to obtain the degradative enzyme mixture; and
treating the lignocellulosic material with the degradative enzyme mixture derived from the Saccharophagus degradans 2-40, thereby degrading the lignin, the hemicellulose, and the cellulose part of the lignocellulosic material to obtain saccharides.
2. The method of claim 1 , wherein the degradative enzyme mixture comprises three or more of the following:
at least one of the compounds listed in FIG. 4 ,
at least one of the compounds listed in FIG. 5 ,
at least one of the compounds listed in FIG. 6 ,
at least one of the compounds listed in FIG. 7 ,
at least one of the compounds listed in FIG. 8 ,
at least one of the compounds listed in FIG. 9 ,
at least one of the compounds listed in FIG. 10 , and
at least one of the compounds listed in FIG. 11 .
3. The method of claim 1 , wherein the degradative enzyme mixture in the treating step is provided in dry form, in a buffer, or in the form of a paste, paint, or micelle.
4. The method of claim 1 , wherein the degradative enzyme mixture in the treating step is in a system comprising cellase, pectinase, and hemicellulase.
5. The method of claim 1 , wherein the degradative enzyme mixture in the treating step is present in a system further comprising metal ions, chelators, detergents, organic ions, inorganic ions, or one or more additional proteins.
6. The method of claim 5 , where the one or more additional proteins are biotin and/or albumin.
7. The method of claim 1 , wherein the treating is conducted in a marine environment.
8. The method of claim 7 , wherein the treating is conducted under water.
9. The method of claim 4 , wherein the degradative enzyme mixture comprises cellulase Cel5F having SEQ ID NO: 13.
10. The method of claim 4 , wherein the degradative enzyme mixture comprises cellulase cel5H having SEQ ID NO: 17.
11. The method of claim 4 , wherein the degradative enzyme mixture comprises cellulase Cel5I having SEQ ID NO: 19.
12. The method of claim 1 , further comprising contacting the saccharides with an ethanologenic microorganism to produce ethanol from the lignocellulosic material.