Aquaretic and natriuretic polypeptides lacking vasodilatory activity
View Patent ↗This document provides aquaretic and natriuretic polypeptides. For example, this document provides polypeptides having aquaretic and/or natriuretic activities. In some cases, a polypeptide provided herein can have aquaretic and natriuretic activities, while lacking the ability to lower blood pressure. This document also provides methods and materials for inducing aquaretic and/or natriuretic activities within a mammal.
1. A natriuretic polypeptide less than 45 amino acid residues in length, wherein said polypeptide consists essentially of, in an order from amino terminus to carboxy terminus:
(a) the sequence set forth in SEQ ID NO:1 ,
(b) the sequence set forth in SEQ ID NO:2, and
(c) the sequence set forth in SEQ ID NO:3.
2. The polypeptide of claim 1 , wherein said polypeptide comprises natriuretic activity.
3. The polypeptide of claim 1 , wherein said polypeptide lacks vasodilatory activity.
4. The polypeptide of claim 1 , wherein said polypeptide is a substantially pure polypeptide.
5. An isolated nucleic acid encoding a natriuretic polypeptide less than 45 amino acid residues in length, wherein said polypeptide consists essentially of, in an order from amino terminus to carboxy terminus:
(a) the sequence set forth in SEQ ID NO:1,
(b) the sequence set forth in SEQ ID NO:2, and
(c) the sequence set forth in SEQ ID NO:3.
6. A vector comprising a nucleic acid encoding a natriuretic polypeptide less than 45 amino acid residues in length, wherein said polypeptide consists essentially of, in an order from amino terminus to carboxy terminus:
(a) the sequence set forth in SEQ ID NO:1,
(b) the sequence set forth in SEQ ID NO:2, and
(c) the sequence set forth in SEQ ID NO:3.
7. A host cell comprising a nucleic acid encoding a natriuretic polypeptide less than 45 amino acid residues in length, wherein said polypeptide consists essentially of, in an order from amino terminus to carboxy terminus:
(a) the sequence set forth in SEQ ID NO:1,
(b) the sequence set forth in SEQ ID NO:2, and
(c) the sequence set forth in SEQ ID NO:3.
8. The host cell of claim 7 , wherein said host cell is a eukaryotic host cell.
9. A pharmaceutical composition comprising a pharmaceutically acceptable carrier and a natriuretic polypeptide less than 45 amino acid residues in length, wherein said polypeptide consists essentially of, in an order from amino terminus to carboxy terminus:
(a) the sequence set forth in SEQ ID NO:1,
(b) the sequence set forth in SEQ ID NO:2, and
(c) the sequence set forth in SEQ ID NO:3.
10. A method for increasing aquaretic and natriuretic activity within a mammal without lowering blood pressure, wherein said method comprises administering to said mammal a natriuretic polypeptide less than 45 amino acid residues in length, wherein said polypeptide consists essentially of, in an order from amino terminus to carboxy terminus:
(a) the sequence set forth in SEQ ID NO:1,
(b) the sequence set forth in SEQ ID NO:2, and
(c) the sequence set forth in SEQ ID NO:3.
11. The polypeptide of claim 1 , wherein the polypeptide comprises the sequence set forth in SEQ ID NO:4.
12. The polypeptide of claim 1 , wherein the polypeptide consists of the sequence set forth in SEQ ID NO:4.