Methods of inhibiting cell death or inflammation in a mammal by administering a BCL protein
View Patent ↗In one aspect the present invention provides methods for inhibiting cell death or inflammation in a mammal, wherein the methods each include the step of administering to a mammal a Bcl protein in an amount sufficient to inhibit cell death or inflammation in the mammal. The invention also provides methods for identifying a Bcl protein that inhibits cell death or inflammation when administered to a mammal.
1. A method for inhibiting inflammation in a mammal suffering from inflammation, comprising administering to the mammal an isolated protein comprising an amino acid sequence at least 90% similar to the amino acid sequence set forth in SEQ ID NO:18 in an amount sufficient to inhibit inflammation in the mammal, wherein the isolated protein is 25 to 175 amino acids in length.
2. The method of claim 1 , wherein the isolated protein comprises the amino acid sequence set forth in SEQ ID NO:18.
3. The method of claim 1 , wherein the isolated protein consists of the amino acid sequence set forth in SEQ ID NO:18.
4. The method of claim 1 , wherein the isolated protein comprises an amino acid sequence at least 90% similar to the amino acid sequence set forth in SEQ ID NO:16.
5. The method of claim 1 , wherein the isolated protein comprises the amino acid sequence set forth in SEQ ID NO:16.
6. The method of claim 1 , wherein the isolated protein consists of the amino acid sequence set forth in SEQ ID NO:16.
7. The method of claim 1 , wherein the isolated protein comprises an amino acid sequence at least 90% similar to the amino acid sequence set forth in SEQ ID NO:5.
8. The method of claim 1 , wherein the isolated protein comprises the amino acid sequence set forth in SEQ ID NO:5.
9. The method of claim 1 , wherein the isolated protein consists of the amino acid sequence set forth in SEQ ID NO:5.
10. The method of claim 1 , wherein the isolated protein comprises an amino acid sequence at least 90% similar to the amino acid sequence set forth in SEQ ID NO:4.
11. The method of claim 1 , wherein the isolated protein comprises the amino acid sequence set forth in SEQ ID NO:4.
12. The method of claim 1 , wherein the isolated protein consist of the amino acid sequence set forth in SEQ ID NO:4.
13. The method of claim 1 , wherein the mammal is suffering from ischemia of an organ or limb.
14. The method of claim 1 , wherein the mammal is suffering from ischemia-repurfusion injury.
15. The method of claim 1 , wherein the isolated protein comprises the BH4 domain of the Bcl-2 family of proteins, and wherein the BH4 domain of the Bcl-2 family of proteins is located in the N-terminal domain of the isolated protein.
16. The method of claim 1 , wherein the isolated protein is administered transdermally.
17. The method of claim 1 , wherein the mammal is suffering from ischemia.
18. The method of claim 1 , wherein the isolated protein is administered intravenously, subcutaneously, intraperitoneally, or transdermally.
19. A method for inhibiting inflammation in a mammal suffering from inflammation, comprising administering to the mammal an isolated protein comprising an amino acid sequence at least 90% similar to the amino acid sequence set forth in SEQ ID NO:16 in an amount sufficient to inhibit inflammation in the mammal.
20. The method of claim 19 , wherein the isolated protein comprises an amino acid sequence at least 96% similar to the amino acid sequence set forth in SEQ ID NO:16.