Patent
The invention provides nucleic acids and polypeptides for nucleic acid polymerases from a thermophilic organism, Thermus brockianus . The invention also provides methods for using these nucleic acids and polypeptides.
1. An isolated nucleic acid polymerase comprising SEQ ID NO:9.
2. An isolated nucleic acid polymerase comprising SEQ ID NO:9 having a G43D mutation that decreases 5′-3′ exonuclease activity relative to the SEQ ID NO:9 nucleic acid polymerase without the G43D mutation.
3. An isolated nucleic acid polymerase comprising SEQ ID NO:9 having a F665Y mutation that reduces discrimination against dideoxynucleotide triphosphates relative to a the SEQ ID NO:9 nucleic acid polymerase without the F665Y mutation.
4. A kit comprising a container containing a nucleic acid polymerase, wherein the nucleic acid polymerase comprises SEQ ID NO:9.
5. The kit of claim 4 further comprising a container containing an unlabeled nucleotide, a labeled nucleotide, a balanced mixture of nucleotides, a chain terminating nucleotide, a nucleotide analog, a buffer solution, a solution containing magnesium, a cloning vector, a restriction endonuclease, a sequencing primer, a solution containing reverse transcriptase, or a DNA or RNA amplification primer.
6. The kit of claim 4 , adapted for performing DNA sequencing, DNA amplification, reverse transcription, RNA amplification or primer extension.