Methods for selective targeting
A selective targeting method is disclosed comprising contacting a library of ligands, particularly a peptide library, with an anti-target to allow the ligands to bind to the anti-target; separating the non-binding ligands from the anti-target bound ligands, contacting the non-binding anti-target ligands with a target allowing the unbound ligands to bind with the target to form a target-bound ligand complex; separating the target-bound ligand complex from ligands which do not bind to the target, and identifying the target-bound ligands on the target-bound ligand complex wherein the target-bound ligands have a K D in the range of about 10 −7 to 10 −10 M. Additionally claimed are the ligands identified according to the method.
1. A method for identifying peptides useful in a cleaning composition comprising the steps of,
(a) contacting a peptide library with an anti-target under conditions favorable for binding between the peptides of the library and the anti-target, wherein the anti-target is a fabric and the peptide library is a phage display library;
(b) separating anti-target unbound peptides from anti-target bound peptides;
(c) contacting the anti-target unbound peptides with a target under conditions favorable for binding between the anti-target unbound peptides and the target to form target-bound peptide complexes, wherein the target is a human body soil on the fabric;
(d) separating the target-bound peptide complexes from anti-target unbound peptides which do not bind to the target; and
(e) identifying the target-bound peptides on the target-bound peptide complexes, wherein the identified target-bound peptides are less than 25 amino acids in length, thereby identifying peptides useful in a cleaning composition.
2. The method according to claim 1 , wherein the cleaning composition is a detergent composition.
3. The method according to claim 1 , wherein the fabric is selected from the group consisting of cotton, wool, silk, polyester, rayon, linen, nylon and blends thereof.
4. The method according to claim 1 , wherein the human body soil is a collar soil.
5. The method according to claim 1 , wherein step (a), (b), (c) or (d) is repeated between 2 to 10 times.
6. The method according to claim 1 , wherein said contacting step is in vitro.
7. The method according to claim 1 , wherein the selectivity ratio of target binding to anti-target binding for the identified target-bound peptides is at least 10:1.
8. The method according to claim 1 , wherein k off of the target-bound peptide complexes is about 10 −4 sec −1 or less.
9. The method according to claim 1 , wherein the identifying step comprises amplifying a nucleic acid coding for the target-bound peptide in a polymerase chain reaction.
10. The method according to claim 1 , wherein the target-bound peptide is not released from the target during the identifying step.
11. The method according to claim 1 , wherein separating said target-bound peptide further includes an acid elution step.
12. The method according to claim 1 , wherein the selectivity ratio of target binding affinity to anti-target binding affinity for the identified target-bound peptides is at least 20:1.
13. The method according to claim 1 , wherein the identified target-bound peptides have a K D of at least about 10 −7 M.