IP Library › Granted Patent US 8,338,157
Granted Patent B2
US 8,338,157 · App. 12/879,428 · Granted Dec 25, 2012

Rationally-designed meganuclease variants of lig-34 and I-crei for maize genome engineering

Assignee: Precision Biosciences, Inc.
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Quick Facts
Patent No.
US 8,338,157
App. No.
12/879,428
Granted
Dec 25, 2012
Kind
B2
Abstract

The invention relates to the field of molecular biology and recombinant nucleic acid technology. In particular, the invention relates to a rationally-designed, non-naturally-occurring meganuclease with altered DNA recognition sequence specificity which recognizes and cleaves a unique DNA site in the maize genome. Disclosed herein are meganucleases which are variants of the I-CreI and LIG- 34 meganucleases. The invention also relates to methods of producing engineered maize plants using such meganucleases.

Claims (3)

1. A rationally-designed meganuclease having the amino acid sequence of SEQ ID NO: 1.

2. A rationally-designed meganuclease having the amino acid sequence of SEQ ID NO: 12.

3. A rationally-designed meganuclease having the amino acid sequence of SEQ ID NO: 13.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 30, 2011
From: JANTZ, DEREK; SMITH, JAMES J.
To: PRECISION BIOSCIENCES, INC.
Reel/Frame 027461/0868 →
Continuity (3)
Continuation PCTUS2009035719 · Mar 2, 2009
Provisional Application 61035613 · Mar 11, 2008
Related Publication 20110113509A1 · May 12, 2011