VMP-like sequences of pathogenic
The present invention relates to DNA sequences encoding Vmp-like polypeptides of pathogenic Borreliae , the use of the DNA sequences in recombinant vectors to express polypeptides, the encoded amino acid sequences, application of the DNA and amino acid sequences to the production of polypeptides as antigens for immunoprophylaxis, immunotherapy, and immunodiagnosis. Also disclosed are the use of the nucleic acid sequences as probes or primers for the detection of organisms causing Lyme disease, relapsing fever, or related disorders, and kits designed to facilitate methods of using the described polypeptides, DNA segments and antibodies.
1. A method of assaying for Borrelia infection comprising:
(a) contacting a sample obtained from a subject with an isolated polypeptide consisting of an epitope of SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:18, SEQ ID NO:19, SEQ ID NO:20, SEQ ID NO:21, SEQ ID NO:22, SEQ ID NO:23, SEQ ID NO:24, SEQ ID NO:25, SEQ ID NO:26, SEQ ID NO:27, SEQ ID NO:28, SEQ ID NO:29, or SEQ ID NO:30; and
(b) determining whether immunologic binding occurs between the isolated polypeptide and an antibody in the sample, wherein immunologic binding is indicative of Borrelia infection.
2. The method of claim 1 , wherein the sample is blood.
3. The method of claim 1 , wherein the sample is urine.
4. The method of claim 1 , wherein the subject is a human.
5. The method of claim 1 , wherein the subject is a dog, deer, or mouse.