Polynucleotides encoding BSL2vcvc-lg
The present invention provides nucleic acids encoding B7-related factors that modulate the activation of immune or inflammatory response cells, such as T-cells. Also provided are expression vectors and fusion constructs comprising nucleic acids encoding B7-related polypeptides, including BSL1, BSL2, and BSL3. The present invention further provides isolated B7-related polypeptides, isolated fusion proteins comprising B7-related polypeptides, and antibodies that are specifically reactive with B7-related polypeptides, or portions thereof. In addition, the present invention provides assays utilizing B7-related nucleic acids, polypeptides, or peptides. The present invention further provides compositions of B7-related nucleic acids, polypeptides, fusion proteins, or antibodies that are useful for the immunomodulation of a human or animal subject.
1. An isolated polynucleotide encoding a fusion protein consisting of a first nucleic acid encoding amino acids 29 to 534 of SEQ ID NO:7 fused to a second nucleic acid sequence encoding the Fc domain of an immunoglobulin.
2. The isolated polynucleotide of claim 1 , wherein said first nucleic acid consists of nucleotides 205 to 1722 of SEQ ID NO:6.
3. An isolated polynucleotide encoding a fusion protein selected from the group consisting of:
(a) an isolated polynucleotide comprising a nucleic acid encoding amino acids 1 to 698 of SEQ ID NO:9;
(b) an isolated polynucleotide comprising a nucleic acid encoding amino acids 2 to 698 of SEQ ID NO:9; and
(c) an isolated polynucleotide comprising a nucleic acid nucleic acid encoding amino acids 29 to 698 of SEQ ID NO:9.
4. The isolated polynucleotide sequence of claim 3 , wherein said polynucleotide of (a) comprises nucleotides 1 to 2094 of SEQ ID NO:8.
5. An isolated polynucleotide encoding a fusion protein selected from the group consisting of:
(a) an isolated polynucleotide comprising a first nucleic acid that encodes amino acids 29 to 465 of SEQ ID NO:7 as contained in plasmid BSL2-4616811 of ATCC Deposit No. PTA-1993 fused to a second nucleic acid sequence encoding the Fc domain of an immunoglobulin; and
(b) an isolated polynucleotide encoding the fusion protein encoded by the BSL2vcvc-Ig cDNA contained in ATCC Deposit No. PTA-4056.
6. The isolated polynucleotide sequence of claim 5 , wherein said polynucleotide of (c) comprises nucleotides 85 to 2094 of SEQ ID NO:8.
7. The isolated polynucleotide sequence of claim 6 , wherein said polynucleotide of (b) comprises nucleotides 4 to 2094 of SEQ ID NO:8.
8. A recombinant vector comprising the isolated polynucleotide of claim 1 , 3 , or 5 .
9. A recombinant host cell comprising the vector of claim 8 .
10. A method of making an isolated polypeptide comprising:
(a) culturing the recombinant host cell of claim 9 under conditions such that said polypeptide is expressed; and
(b) recovering said polypeptide.
11. An isolated polynucleotide comprising a polynucleotide encoding a fusion protein consisting of polynucleotides encoding the extracellular domain of SEQ ID NO:7 fused to a heterologous nucleic acid sequence encoding the Fc domain of an immunoglobulin, wherein said extracellular domain is amino acids 2 to 465 of SEQ ID NO:7.
12. An isolated polynucleotide comprising a polynucleotide encoding a fusion protein consisting of polynucleotides encoding the mature extracellular domain of SEQ ID NO:7 fused to a heterologous nucleic acid sequence encoding the Fc domain of an immunoglobulin, wherein said mature extracellular domain is amino acids 29 to 465 of SEQ ID NO:7.
13. The isolated polynucleotide according to claim 1 , 3 , 5 , 11 or 12 , wherein said immunoglobulin is IgG.
14. A recombinant vector comprising the isolated polynucleotide of claim 11 , or 12 .
15. A recombinant host cell comprising the vector of claim 14 .
16. A method of making an isolated polypeptide comprising:
(a) culturing the recombinant host cell of claim 15 under conditions such that said polypeptide is expressed; and
(b) recovering said polypeptide.