Biomimetic iron-oxide-containing lipoprotein and related materials
View Patent ↗An engineered lipoprotein including (a) a core particle or a plurality of core particles, each core particle has (i) an inner part comprising a hydrophilic active agent and a hydrophilic portion of an amphiphilic cholesterol and (ii) an outer part including a hydrophobic portion of the amphiphilic cholesterol, (b) a layer surrounding the core particle or a plurality of core particles, the layer includes a phospholipid, (c) an apoprotein associated with the layer, and optionally, (d) a homing molecule associated with at least one of the apoprotein or the phospholipid.
1. An engineered lipoprotein comprising:
(a) a core particle or a plurality of core particles, each core particle having (i) an inner part consisting of one or more hydrophilic active agents and a hydrophilic portion of an amphiphilic cholesterol and (ii) an outer part comprising a hydrophobic portion of the amphiphilic cholesterol;
(b) a layer surrounding the core particle or the plurality of core particles, the layer comprising a phospholipid;
(c) an apoprotein associated with the layer, and optionally,
(d) a homing molecule associated with at least one of the apoprotein or the phospholipid.
2. The engineered lipoprotein of claim 1 , wherein the one or more hydrophilic active agents are a diagnostic agent.
3. The engineered lipoprotein of claim 2 , wherein the diagnostic agent is a magnetic resonance imaging (MRI) contrast agent.
4. The engineered lipoprotein of claim 3 , wherein the MRI contrast agent comprises Fe, Co, Mn, Ni, or Cr.
5. The engineered lipoprotein of claim 4 , wherein the MRI contrast agent is iron oxide.
6. The engineered lipoprotein of claim 1 , wherein the hydrophilic portion of the amphiphilic cholesterol is at least one of (a) an anionic group selected from at least one of sulfate, sulfonate or carboxylate anions, (b) a cationic group selected from at least one of quaternary ammonium cations, or (c) a hydrophilic nonionic group selected from at least one of poly(ethylene glycol), alkyl poly(ethylene oxide) and alkyl poly(glucoside).
7. The engineered lipoprotein of claim 6 , wherein the amphiphilic cholesterol is a member selected from the group consisting of cholesteryl 3β-N-(dimethylaminoethyl)carbamate hydrochloride, cholic acid, 3-cholesteryloxycarbonyl pentanoic acid, cholesterol-poly(ethylene glycol), cholesterol-poly(ethylene oxide) and cholesterol-poly(glucoside).
8. The engineered lipoprotein of claim 1 , wherein the phospholipid is a member selected from the group consisting of phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, phosphatidic acid, phosphatidylglycerol, and cardiolipin.
9. The engineered lipoprotein of claim 1 , wherein the apoprotein is a member selected from the group consisting of apoprotein A1, A2, A4, B48, B100, C1, C2, C3, D, and E.
10. The engineered lipoprotein of claim 1 , wherein the inner part of the core particle consists of a plurality of hydrophilic active agents and a hydrophilic portion of an amphiphilic cholesterol.