Mobilization of hematopoietic stem cells
Methods, processes, uses, and pharmaceutical compositions are provided herein for mobilizing hematopoietic progenitor cells and/or cancer stem cells from bone marrow into peripheral blood, comprising the administration of an effective amount of an inhibitor of GTPases, such as a Cdc-42 specific inhibitor alone or in combination with one or more additional agents. Specifically, methods are disclosed for mobilizing hematopoietic stem cells into a subject's peripheral blood. In particular, embodiments of the method involve specific inhibition of the Cdc42 GTPase to increase the numbers of hematopoietic stem cells into a subject's peripheral blood of a subject.
1. A method for mobilizing peripheral blood precursor cells from bone marrow into peripheral blood comprising: administering to a subject in need of treatment an effective amount of at least one Cdc42-specific inhibitor, wherein the Cdc42-specific inhibitor is CASIN.
2. A method for facilitating hematopoietic reconstitution of peripheral blood precursor cells in a subject's hematopoietic organs, comprising: a) administering to the subject an effective amount of at least one Cdc42-specific inhibitor in the precursor cells; b) isolating mobilized peripheral blood precursor cells from the peripheral circulation of the subject; and c) infusing the isolated mobilized peripheral blood precursor cells into the subject, wherein the Cdc42-specific inhibitor is CASIN.