Method to identify disease resistant quantitative trait loci in soybean and compositions thereof
The present invention is in the field of plant breeding and genetics, particularly as it pertains to the genus, Glycine . More specifically, the invention relates to a method for screening soybean plants containing one or more quantitative trait loci for disease resistance, species of Glycine having such loci and methods for breeding for and screening of Glycine with such loci. The invention further relates to the use of exotic germplasm in a breeding program.
1. A method for producing an Asian Soybean Rust (ASR) resistant soybean plant comprising:
selecting a plant using marker assisted selection from a plurality of soybean plants by detecting a molecular marker in linkage group C2, wherein said plurality of soybean plants is derived from a soybean plant which carries resistance to ASR, and wherein said molecular marker is from a chromosome region encompassing an ASR resistance locus and a SNP marker selected from the group consisting of NS0119715, NS0118897, NS0130920, NS0099746, NS0125408, NS0126598, NS0103749, NS0128378, NS0123747, NS0096829, NS0098902, NS0099529, NS0097798, NS0137477, NS0136101, NS0095322, and NS0098982; and
generating a progeny plant from said selected plant.
2. The method of claim 1 , wherein said soybean plant which carries resistance to ASR comprises ASR resistance locus 3.
3. The method of claim 1 , wherein said SNP marker is selected from the group consisting of NS0118897, NS0130920, NS0099746, NS0125408, NS0126598, NS0103749, NS0128378, NS0123747, NS0096829, NS0098902, NS0099529, NS0097798, NS0137477, NS0136101, and NS0095322.
4. The method of claim 1 , wherein said SNP marker is selected from the group consisting of NS0130920, NS0099746, NS0125408, NS0126598, NS0103749, NS0128378, NS0123747, NS0096829, NS0098902, NS0099529, NS0097798, NS0137477, and NS0136101.
5. The method of claim 1 , wherein said SNP marker is selected from the group consisting of NS0099746, NS0125408, NS0126598, NS0103749, NS0128378, NS0123747, NS0096829, NS0098902, NS0099529, NS0097798, and NS0137477.
6. The method of claim 1 , wherein said SNP marker is selected from the group consisting of NS0125408, NS0126598, NS0103749, NS0128378, NS0123747, NS0096829, NS0098902, NS0099529, and NS0097798.
7. The method of claim 1 , wherein said soybean plant which carries resistance to ASR is obtainable from a soybean plant selected from the group consisting of PI462312, PI200487, PI547878, and AVRDC-8.
8. The method of claim 1 , wherein said molecular marker is selected from the group consisting of NS0119715, NS0118897, NS0130920, NS0099746, NS0125408, NS0126598, NS0103749, NS0128378, NS0123747, NS0096829, NS0098902, NS0099529, NS0097798, NS0137477, NS0136101, NS0095322, and NS0098982.
9. The method of claim 8 , wherein said molecular marker is selected from the group consisting of NS0137477, NS0103749, NS0118897, NS0119715, and NS0130920.
10. The method of claim 9 , wherein said molecular marker is NS0137477.
11. The method of claim 1 , wherein said progeny plant further comprises one or more ASR resistance loci selected from the group consisting of ASR resistance loci 1, 2 and 4-13.
12. The method of claim 1 , wherein said progeny plant is resistant to the pathogen Phakopsora meibomiae.
13. The method of claim 1 , wherein said progeny plant is resistant or immune to ASR.
14. A plant produced by the method of claim 1 .
15. The method of claim 1 , wherein said progeny plant is resistant to the pathogen Phakopsora pachyrhizi.
16. The method of claim 11 , wherein said ASR resistance locus 1 is localized to linkage group G and is mapped within 10 centimorgans or less from one or more SNP markers selected from the group consisting of NS0093250, NS0119710, NS0103004, NS0099454, NS0102630, NS0102915, NS0102913, NS0123728, NS0129943, NS0102168, NS0092723, NS0098177, NS0127343, and NS0101121, said ASR resistance loci 5-9 are localized to chromosome 13 and are mapped within 10 centimorgans or less from SNP marker NS0103033, and said ASR resistance loci 10-13 are localized to chromosome 13 and are mapped within 10 centimorgans or less from SNP marker NS0124935.
17. The method of claim 16 , wherein each of said 10 centimorgans or less is 5 centimorgans or less.
18. The method of claim 1 , wherein said SNP marker is selected from the group consisting of NS0126598, NS0103749, NS0128378, NS0123747, NS0096829, NS0098902, and NS0099529.
19. The method of claim 1 , wherein said SNP marker is selected from the group consisting of NS0103749, NS0128378, NS0123747, NS0096829, and NS0098902.
20. The method of claim 1 , wherein said SNP marker is NS0137477.