High frequency deflection measurement of IR absorption
An AFM based technique has been demonstrated for performing highly localized IR spectroscopy on a sample surface. Significant issues as to size, cost of implementation, and repeatability/robustness of results exist in commercializing the technique. The invention addresses many of these issues thereby producing a version of the analytical technique that can be made generally available to the scientific community.
1. An instrument for measuring infrared absorption of a sample on a sub-wavelength scale comprising:
a benchtop source of infrared radiation, adapted to be modulated at a pulse repetition rate in excess of 100 Hz, optically coupled to a region of the sample;
a probe including a tip with a sub-micron end radius that interacts with the sample and to respond to infrared radiation absorbed by the sample; and,
a detector for measuring the probe response at one or more wavelengths of the infrared radiation,
wherein the instrument creates a spatially resolved map indicative of absorbed infrared radiation over the region of the sample with at least 100×100 pixels in less than thirty minutes.
2. The instrument of claim 1 wherein the spatially resolved map has a spatial resolution of less than 1 um.
3. The instrument of claim 1 wherein each pixel in the spatially resolved map uses the probe response from at least ten pulses from the benchtop source of infrared radiation.
4. A method of measuring a spectrum of infrared absorption of a sample on a sub-wavelength scale comprising the steps of:
optically coupling a benchtop source of coherent infrared radiation to a region of a sample;
modulating the intensity of the radiation incident on the sample at a frequency in excess of 100 Hz;
interacting a cantilever probe with a tip with a sub-micron end radius with the sample such that the probe responds to infrared radiation absorbed by the sample;
detecting a probe response to modulated infrared radiation absorbed by the sample at one or more wavelengths;
generating relative lateral motion between the probe and the sample; and,
creating a spatial map from the detected probe response indicative of infrared absorption of a region of the sample, wherein the map has a pixel density of at least 100×100 pixels and the map is completed in a time of less than thirty minutes.
5. The method of claim 4 wherein the radiation from the benchtop source is selected to have at least one wavelength corresponding to an absorption band of the sample.
6. The method of claim 4 further comprising the steps of attaching an infrared tag to a plurality of molecules and introducing the tagged molecules into the sample, wherein the spatially resolved map of infrared absorption is indicative of the spatial uptake of the tagged molecules by the sample.
7. The method of claim 6 wherein the sample is a biological cell.
8. The method of claim 4 wherein the spatial map is used to create a spatially resolved map of chemical composition with sub-micron resolution.
9. The method of claim 4 further comprising sweeping the wavelength of the infrared radiation supplied by the benchtop source.
10. The method of claim 9 further completing the sweeping step in less than 100 seconds.
11. The method of claim 9 further comprising sweeping the wavelength of the infrared radiation supplied by the benchtop source over a range of at least 100 cm −1 .