Nitrate reductase fusion proteins and uses thereof
The present invention relates to a novel fusion protein comprising a nitrate reductase (NR) and a truncated hemoglobin N (trHbN) domain. The fusion protein may be used for bioremediation of nitric oxide.
1. An isolated fusion protein comprising a nitrate reductase (NR) and a truncated hemoglobin N (trHbN) domain, wherein the fusion protein comprises a polypeptide having an amino acid sequence at least 90% identical to SEQ ID NO: 3 or 5.
2. The isolated fusion protein of claim 1 , wherein the fusion protein is capable of reducing nitric oxide.
3. The isolated fusion protein of claim 1 , wherein the fusion protein is capable of converting nitric oxide to nitrate and nitrite.
4. The isolated fusion protein of claim 1 , wherein the fusion protein comprises a polypeptide having the amino acid sequence of SEQ ID NO: 3 or 5.
5. The isolated fusion protein of claim 1 , wherein the fusion protein is derived from a raphidophyte.
6. The isolated fusion protein of claim 5 , wherein the raphidophyte is selected from the group consisting of genus Heterosigma , and Chattonella.
7. The isolated fusion protein of claim 5 , wherein the raphidophyte is selected from the group consisting of species Heterosigma akashiwo , and Chattonella subsalsa.
8. A composition for reducing nitric oxide, comprising an effective amount of an isolated fusion protein, wherein the fusion protein comprises a nitrate reductase (NR) and a truncated hemoglobin N (trHbN) domain, wherein the fusion protein comprises a polypeptide having an amino acid sequence at least 90% identical to SEQ ID NO: 3 or 5.
9. The composition of claim 8 , wherein the fusion protein is capable of converting nitric oxide to nitrate and nitrite.
10. The composition of claim 8 , wherein the fusion protein comprises a polypeptide having the amino acid sequence of SEQ ID NO: 3 or 5.
11. The composition of claim 8 , wherein the fusion protein is derived from a raphidophyte.
12. The composition of claim 11 , wherein the raphidophyte is selected from the group consisting of genus Heterosigma , and Chattonella.
13. The composition of claim 11 , wherein the raphidophyte is selected from the group consisting of species Heterosigma akashiwo , and Chattonella subsalsa.
14. A method for reducing nitric oxide, comprising applying an effective amount of a fusion protein, wherein the fusion protein comprises a nitrate reductase (NR) and a truncated hemoglobin N (trHbN) domain, and wherein the fusion protein comprises a polypeptide having an amino acid sequence at least 90% identical to SEQ ID NO: 3 or 5.
15. The method of claim 14 , wherein the fusion protein converts nitric oxide to nitrate and nitrite.
16. The method of claim 14 , wherein the fusion protein comprises a polypeptide having the amino acid sequence of SEQ ID NO: 3 or 5.
17. The method of claim 14 , wherein the fusion protein is derived from a raphidophyte.
18. The method of claim 17 , wherein the raphidophyte is selected from the group consisting of genus Heterosigma , and Chattonella.
19. The method of claim 17 , wherein the raphidophyte is selected from the group consisting of species Heterosigma akashiwo , and Chattonella subsalsa.
20. The method of claim 14 , wherein the fusion protein reduces nitric oxide by at least 50%.