Subcutaneous glucose electrode
A small diameter flexible electrode designed for subcutaneous in vivo amperometric monitoring of glucose is described. The electrode is designed to allow “one-point” in vivo calibration, i.e., to have zero output current at zero glucose concentration, even in the presence of other electroreactive species of serum or blood. The electrode is preferably layered, with the layers serially deposited within a recess upon the tip of a polyamide insulated gold wire. A first glucose concentration-to-current transducing layer can be overcoated with an electrically insulating and glucose flux limiting layer (second layer) on which, optionally, an immobilized interference-eliminating horseradish peroxidase based film is deposited. An outer layer is preferably biocompatible.
1. An analyte sensor comprising:
one or more working electrodes;
an analyte-responsive sensing layer associated with and in electrical contact with the one or more working electrodes, the sensing layer comprising an enzyme and a polymer selected from the group consisting of poly(vinylimidazole) and poly(vinyl pyridine); and
a polymeric diffusion-limiting layer disposed on and bound to the analyte-responsive sensing layer,
wherein at least a portion of the sensor is subcutaneously positioned in a subject.
2. The analyte sensor of claim 1 , wherein the analyte is glucose and the enzyme is glucose oxidase.
3. The analyte sensor of claim 2 , wherein the analyte sensor has a 10% to 90% response time of not more than about 30 second at a glucose concentration of about 20 mM.
4. The analyte sensor of claim 2 , wherein sensor response to glucose is close to linear through a glucose concentration range of 2 to 20 mM.
5. The analyte sensor of claim 2 , wherein the analyte sensor has substantially no signal output when concentration of glucose is zero.
6. The analyte sensor of claim 1 , wherein the enzyme is covalently bound to the polymer.
7. The analyte sensor of claim 1 , the sensing layer further comprising a redox compound.
8. The analyte sensor of claim 7 , wherein the redox compound is covalently bound to the polymer.
9. The analyte sensor of claim 8 , wherein the enzyme is covalently bound to the polymer.
10. The analyte sensor of claim 1 , wherein the polymeric diffusion-limiting layer limits the rate of analyte transport to the sensing layer.
11. The analyte sensor of claim 1 , further comprising one or more additional layers.
12. The analyte sensor of claim 11 , wherein the one or more additional layers comprises at least one of a biocompatible layer or an interferant eliminating layer.
13. The analyte sensor of claim 1 , wherein the sensor comprises a first portion and a second portion, wherein the first portion is positioned external to the subject and the second portion is positioned subcutaneously in the subject.
14. The analyte sensor of claim 13 , wherein the first portion is connected to a device for measurement of the electrical signal generated by the sensor.
15. The analyte sensor of claim 13 , wherein the second portion of the analyte sensor has a width of no more than about 0.29 mm.
16. The analyte sensor of claim 1 , wherein the sensor has substantially no signal output when the concentration of glucose is zero.
17. The analyte sensor of claim 1 , wherein the sensor comprises at least two electrodes and at least one of the electrodes is a working electrode and at least one other electrode is a reference electrode, a counter electrode or a combined reference and counter electrode.
18. The analyte sensor of claim 1 , wherein one or more working electrodes is a wire.
19. The analyte sensor of claim 1 , wherein the sensor is flexible and provides an electrical signal that is substantially insensitive to relative motion between the implanted portion of the sensor and tissue surrounding the implanted portion of the sensor.
20. The analyte sensor of claim 1 , wherein the sensor has an in vivo glucose sensitivity of from about 10 pA/mg/dL to about 250 pA/mg/dL.
21. The analyte sensor of claim 20 , wherein the in vivo glucose sensitivity does not change by more than about ±5% for 72 hours of operation at 37° C.
22. The analyte sensor of claim 1 , wherein the analyte-responsive sensing layer and the polymeric diffusion-limiting layer are formed in situ on the working electrode.
23. The analyte sensor of claim 1 , wherein the analyte sensor comprises a wire.
24. The analyte sensor of claim 1 , wherein the working electrode has a width of no more than about 0.25 mm.
25. The analyte sensor of claim 1 , wherein the analyte sensor measures an analyte concentration in a host such that at least 80% of measured sensor values from the analyte sensor are within 20% of actual analyte values.
26. An analyte sensor comprising:
an electrode comprising a metal wire;
an analyte-responsive sensing layer associated with the wire, the sensing layer comprising an enzyme and a polymer selected from the group consisting of poly(vinylimidazole) and poly(vinyl pyridine); and
a polymeric diffusion-limiting layer disposed on and bound to the analyte responsive-sensing layer.
27. The analyte sensor of claim 26 , wherein the analyte sensor measures an analyte concentration in a host such that at least 80% of measured sensor values from the analyte sensor are within 20% of actual analyte values.