Medical substitute membrane, use thereof, and method for repair of membrane tissue in living body
The dried amniotic membrane is produced by drying a fresh amniotic membrane, which envelopes an embryo of an animal including human, and can be used as a substitute membrane for a membrane tissue in a living body; the dried amniotic membrane is dehydrated and dried so that the dried amniotic membrane can be stored in a dry air in a sterile state; when hydrated again by immersing in water or a buffer solution, the amniotic membrane still has an epithelial cell, a basement membrane and a connective tissue which constitute the fresh amniotic membrane. The dried amniotic membrane is useful as a medical substitute membrane for a membrane tissue in a living body such as a dura mater, a meninx, a pericardium, a pleura and a peritoneum.
1. A method for repair of a membrane tissue in a living body, comprising:
a step of setting a fresh amniotic membrane, which envelopes an embryo of an animal, in a processing tank;
a step of drying the fresh amniotic membrane by a heating means while reducing an inner pressure of the processing tank to produce a dry amniotic membrane that still has an epithelial cell, a basement membrane, and a connective tissue; and
a step of repairing a membrane tissue of a wound area of the living body, in which the dry amniotic membrane is put on, embedded into, or fills the wound area;
wherein the dry amniotic membrane is used to repair a dura mater of the living body.
2. The method according to claim 1 ,
wherein a process of reducing the inner pressure of the processing tank and a process of increasing the inner pressure until reaching the atmospheric pressure are alternately repeated in said drying step.
3. The method according to claim 1 ,
wherein the heating means used in said drying step is at least one of a far-infrared ray heater and a microwave irradiator.
4. The method according to claim 1 , wherein the fresh amniotic membrane is heated at a temperature of 50° C. or lower in said drying step.
5. The method according to claim 1 ,
wherein the dry amniotic membrane is rehydrated by immersing in water or a buffer solution, and the rehydrated amniotic membrane is put on, embedded into, or fills the wound area so as to repair the membrane tissue of the wound area.
6. The method according to claim 1 , wherein the animal is a human.