Cell culture container and cell culture method
A cell culture chamber and a cell culture method that are capable of effectively constructing an intercellular network in a culture space are provided. A cell culture chamber ( 10 )according to the present invention is a cell culture chamber ( 10 )including a plurality of microchambers ( 11 ) formed on a surface thereof, characterized in that convex portions (side walls 12 ) that partition the microchambers ( 11 ) adjacent to each other are formed to prevent cells from being adhered to upper surfaces of the convex portions. Consequently, cells can be cultured exclusively within the microchambers ( 11 ), and an intercellular network can be constructed effectively.
1. A cell culture method for culturing cells only within microchambers, the method comprising:
injecting cells into the microchambers formed in a cell culture chamber, and
culturing the cells only within the microchambers, wherein the cells do not adhere to upper surfaces of convex portions,
wherein the cell culture chamber comprises a plurality of microchambers formed on a surface of the cell culture chamber, and wherein the convex portions that partition the microchambers adjacent to one another are formed such that cells are contained in the microchambers, thereby preventing the cells from adhering to the upper surfaces of the convex portions,
wherein each of the upper surfaces of the convex portions of the cell culture chamber has a short side width of from 0.5 to 15 μm, and each of the convex portions has a height more than three times of the short side width, and
wherein the cells are liver cells.
2. The cell culture method according to claim 1 , wherein the convex portions have a multi-stage structure and the cells do not adhere to an upper surface of each stage during culturing.
3. The cell culture method according to claim 1 , wherein each of the convex portions has a height of from 30 to 300 μm.
4. The cell culture method according to claim 1 , wherein 50% or more of an upper portion in a height direction of the convex portions has an angle of 80° to 90° formed between a bottom surface of the microchamber and each side surface of the convex portions.
5. The cell culture method according to claim 1 , wherein each of the microchambers has a bottom surface area of 6.25×10 −4 mm 2 to 0.563 mm 2 .
6. The cell culture method according to claim 1 , wherein a bottom surface of each of the microchambers has a major axis which is 1 to 1.5 times a minor axis of the bottom surface.
7. The cell culture method according to claim 1 , wherein a bottom surface of each of the microchambers has a major axis which is 1.5 to 50 times a minor axis of the bottom surface, and migration properties of cells are evaluated.
8. The cell culture method according to claim 1 , wherein the microchambers communicate with at least one adjacent microchamber.
9. The cell culture method according to claim 8 , wherein an opening for allowing the microchambers to communicate with the at least one adjacent microchamber has a width of 1 to 25 μm.
10. The cell culture method according to claim 1 , wherein an area having micropatterns is surface treated.
11. The cell culture method according to claim 10 , wherein an integrated layer film formed by the surface treatment has two or more layers comprising at least one layer of an inorganic film and at least one layer of an organic film.