Beam shaping without introducing divergence within a light beam
An optical beam shaper comprises a polarization-dependent phase adjustment member which applies a phase pattern of equal magnitude and opposite sign to two orthogonal polarization states. In a preferred embodiment the beamer shaper is a dif tractive element made of a birefringent material, such as a photo-polymerizable liquid crystal polymer frozen in a uniaxial alignment, said dif tractive element comprising a plurality of zones, each zone having a stepped thickness defining a plurality of steps. The beam shaper can be used to introduce astigmatism to a polarized light beam or to undo the astigmatism to a beam with an orthogonal polarization state. The beam shaper is advantageously used within a detection device, such as a fluorescence scanner.
1. An optical system for illuminating a sample comprising:
a light source;
an optical beam shaper for transforming a collimated beam of light emitted by the light source into an illumination beam, the optical beam shaper comprising a polarization-dependent phase adjustment member for applying a phase pattern of equal magnitude and opposite sign to two orthogonal polarization states;
an imaging system for illuminating the sample with the illumination beam;
a first detector for detecting light reflected from the sample; and
a polarization adjustment arrangement between the optical beam shaper and the sample, wherein the light reflected from the sample passes through the polarization adjustment arrangement and the optical beam shaper before detection by the first detector.
2. An optical system as claimed in claim 1 , further comprising a controller for controlling the imaging system based on analysis of the light detected by the first detector.
3. A detection device, comprising:
the optical system as claimed in claim 1 ;
an optical collection arrangement for collecting light emitted from the sample and generated by the illumination beam; and
a second detector for detecting the collected light.
4. A detection device as claimed in claim 3 , wherein the imaging system and the optical collection arrangement share an excitation/collection lens.
5. An optical system as claimed in claim 1 , wherein the optical beam shaper comprises a diffractive element formed from birefringent material.
6. An optical system as claimed in claim 5 , wherein the diffractive element comprises a plurality of zones, each zone of the plurality of zones comprising a stepped thickness defining a plurality of steps.
7. An illumination method for illuminating a sample, comprising the steps of:
generating a collimated light beam using a light source;
transforming the collimated light beam into a transformed light beam by applying a phase pattern of equal magnitude and opposite sign to two orthogonal polarization states;
passing the transformed light beam through a polarization adjustment arrangement generating an illumination light beam; and
illuminating the sample with the illumination light beam using an imaging system.
8. An illumination method as claimed in claim 7 , further comprising the steps of:
passing a reflected light beam emanating from the illumination light beam reflected from the sample through the polarization adjustment arrangement;
passing the re-transformed light beam to a first detector, and controlling the imaging system based on analysis of the re-transformed light beam detected by the first detector.