Promoter and a production method for L-lysine using the same
Disclosed are a nucleic acid molecule of Corynebacterium glutamicum origin, having an improved promoter activity, which is operably linked to operon encoding aspartate kinase and aspartate semialdehyde dehydrogense, a vector containing the same, a transformant transformed with the vector, and a method for the production of L-lysine using the transformant.
1. An isolated nucleic acid molecule comprising nucleotides 1-353 of SEQ ID NO:2, having promoter activity for expression.
2. A vector comprising the nucleic acid molecule of claim 1 .
3. The vector according to claim 2 , which is pDZ-lysCP1 having a genetic map of FIG. 2 .
4. A transformant transformed with the vector of claim 2 , wherein the transformant is a prokaryote.
5. The transformant according to claim 4 , wherein the prokaryote is a Corynebacterium or Brevibacterium.
6. The transformant according to claim 4 , which is named CA01-0135, and deposited with Accession No. KCCM10919P.
7. The transformant according to claim 4 , wherein the nucleic acid molecule of claim 1 having promoter activity is incorporated into a genome of the transformant through homologous recombination.
8. The transformant according to claim 4 , harboring the nucleic acid molecule of claim 1 in a plasmid.
9. A method for producing lysine, comprising fermenting the transformant of claim 4 .
10. The method according to claim 9 , further comprising collecting the lysine generated during the fermentation.